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R4530

Anti-Rab21 (C-terminal) antibody produced in rabbit

Name: Anti-Rab21 (C-terminal) antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-RAB21, member RAS oncogene family

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关于此项目

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

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属性

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~25 kDa

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using whole extract of human HeLa cells, indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 and rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin, western blot: 5-10 μg/mL using whole extract of human A431 cells

UniProt accession no.

Q9UL25

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB21(23011)
mouse ... Rab21(216344)
rat ... Rab21(299799)

说明

General description

Ras-related protein Rab-21 (Rab21), a close homolog of Rab5, is ubiquitously expressed in human tissues. It localizes mainly to the early endocytic pathway. Rab21 is mapped to human chromosome 12q21.1 It belongs to the Rab family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions, C-terminal hypervariable domains with a cysteine motif.

Immunogen

synthetic peptide corresponding to amino acid residues 203-216 of human RAB21, with an added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat and mouse.

Application

Anti-Rab21 (C-terminal) antibody produced in rabbit may be used in:

immunoblotting
immunoprecipitation
immunofluorescence

Biochem/physiol Actions

Anti-Rab21 (C-terminal) recognizes human, rat, and mouse Rab21.

Ras-related protein Rab-21 (Rab21) and Rab5 are integrin-associated proteins, acting as positive regulators of integrin traffic. The association of Rab21 with integrins modulates cell adhesion and migration. It is also involved in endosome morphology and function. Rab21 is associated with squamous carcinoma and its expression is elevated in glioma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extendedstorage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

10 - Combustible liquids

闪点 (F)

Not applicable

闪点 (°C)