Merck
CN
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文件

R4533

Sigma-Aldrich

RNAzol® RT

For processing total and small RNA from human, animal, plant, bacterial,and viral samples

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别名:
single step RNA isolation, single step RNA purification, total RNA isolation, total RNA purification
NACRES:
NA.52

质量水平

100

用途

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue (or)

相关类别

一般描述

核糖核酸逆转录酶是一种快速方便的试剂，用于从人、动物、植物、酵母、细菌和病毒来源的生物样品中一步分离总核糖核酸和小核糖核酸。通过一种方便的单步液相分离可以从脱氧核糖核酸、蛋白质、多糖和其他分子中分离出核糖核酸。核糖核酸®逆转录酶可用于分离核糖核酸和微小核糖核酸的分离部分，或分离总核糖核酸，在单一部分中包含所有种类的核糖核酸。

该产品是硫氰酸胍和苯酚在单相溶液中的混合物，在组织样品均质化或裂解时有效溶解脱氧核糖核酸、核糖核酸和蛋白质。向混合物中加入水可以沉淀出脱氧核糖核酸、蛋白质、多糖和其他分子，然后通过离心将其除去。然后通过醇沉淀、洗涤和溶解分离核糖核酸。氯仿诱导的相分离是不必要的。1毫升®逆转录酶足以从高达100毫克的组织或10e7细胞中分离出核糖核酸。

这是分离总核糖核酸和小核糖核酸最有效的方法之一，这种方法从新鲜组织或细胞开始，在室温下不到1小时即可完成。该方法对于分离所有类型的核糖核酸分子非常有效：大核核糖核酸、核糖核酸、核糖核酸、小核糖核酸和微小核糖核酸。得到的核糖核酸是完整的、很少或没有污染的脱氧核糖核酸和蛋白质。

应用

分离的核糖核酸可用于Northern blot、核糖核酸酶保护分析、微阵列、聚合酶链反应和其他分子生物学应用。
核糖核酸逆转录酶是一种高效的试剂，用于从人、动物、植物、细菌和病毒来源的样品中一步分离总核糖核酸和小核糖核酸。基于该试剂的核糖核酸分离方法在室温下进行，不使用氯仿进行相分离，并且允许在逆转录聚合酶链反应中使用，而不需要脱氧核糖核酸酶处理。

特点和优势

  • 从生物样品中分离微核糖核酸和总核糖核酸
  • 高产量和高质量的分离核糖核酸
  • 从新鲜组织或细胞开始，在室温下不到一小时内完成

其他说明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

法律信息

RNAZol is a registered trademark of Molecular Research Center, Inc.

警示用语：

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

闪点(°F)

230.0 °F

闪点(°C)

110 °C

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T1503
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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What color is Product R4533, RNAzol® RT?

    Product R4533, RNAzol® RT is a blue solution at both 4°C and room temperature.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

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The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens.
Antonissen G
PLoS ONE, 9(9), e109775-e109775 (2014)
Andrea Miccoli et al.
Marine environmental research, 127, 137-147 (2017-04-17)
Natural/synthetic Endocrine Disrupting Chemicals (EDCs) may display estrogenic activity and a lower potency than 17β-estradiol. Nonetheless, their concentrations and additive effects can affect the endocrine system and reproductive processes related to the Hypothalamic-Pituitary-Gonadal (HPG) axis. Because of their persistence in
Paulina Andryka-Dudek et al.
International journal of molecular sciences, 20(5) (2019-03-01)
Short-term (3 h) treatment of embryos isolated from dormant apple (Malus domestica Borkh.) seeds with NO donors stimulates their transition from dormancy to germination. Seed dormancy is maintained by ABA, while germination is controlled mainly by gibberellins (GAs) and jasmonic
Arturo Vargas et al.
Zebrafish, 15(4), 404-419 (2018-03-29)
A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards. Due to minimal environmental impact, compared with most conventional
Arinder K Arora et al.
Genes, 12(3) (2021-04-04)
Insect pest control by RNA interference (RNAi)-mediated gene expression knockdown can be undermined by many factors, including small sequence differences between double-stranded RNA (dsRNA) and the target gene. It can also be compromised by effects that are independent of the
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商品

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

基因组DNA样品纯化和质量评估

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。

实验方案

MystiCq® MicroRNA® Quantitation System

Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents

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