R4883
试卤灵 β-D-吡喃半乳糖苷
~95%, powder
别名:
3-尼罗红 7-(β-D-吡喃半乳糖苷）
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关于此项目
经验公式（希尔记法）:
C18H17NO8
化学文摘社编号:
分子量:
375.33
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
4335306
产品名称
试卤灵 β-D-吡喃半乳糖苷, ~95%
InChI
1S/C18H17NO8/c20-7-14-15(22)16(23)17(24)18(27-14)25-9-2-4-11-13(6-9)26-12-5-8(21)1-3-10(12)19-11/h1-6,14-18,20,22-24H,7H2/t14-,15+,16+,17-,18-/m1/s1
SMILES string
OC[C@H]1O[C@@H](Oc2ccc3N=C4C=CC(=O)C=C4Oc3c2)[C@H](O)[C@@H](O)[C@H]1O
InChI key
QULZFZMEBOATFS-DISONHOPSA-N
assay
~95%
form
powder
solubility
DMSO: 20 mg/mL, clear, orange to red
storage temp.
−20°C
Quality Level
General description
试卤灵-D-吡喃半乳糖苷是一种非荧光化合物，颜色为橙黄色。它可被β-半乳糖苷酶 (β-（＆＃946; -Gal）水解，产生荧光试卤灵。
Packaging
无底玻璃瓶。内含物在插入的融合锥体内。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
涉药品监管产品
此项目有
Microchip device for performing enzyme assays
Hadd AG, et al.
Analytical Chemistry, 69(17), 3407-3412 (1997)
Immobilized enzyme kinetics analyzed by flow-through microfluorimetry: resorufin-beta-D-galactopyranoside as a new fluorogenic substrate for beta-galactosidase
Hofmann J and Sernetz M
Analytica Chimica Acta, 163, 67-72 (1984)
Seung-Yong Jung et al.
Langmuir : the ACS journal of surfaces and colloids, 24(9), 4439-4442 (2008-03-26)
A device with femtoliter-scale chambers and controlled reaction initiation was developed for single-molecule enzymology. Initially separated substrate and enzyme streams were rapidly mixed in a microfluidic device and encapsulated in an array of individual microreactors, allowing for enzyme kinetics to
Yuliang Xie et al.
Analytical chemistry, 84(17), 7495-7501 (2012-08-14)
In this work we present an acoustofluidic approach for rapid, single-shot characterization of enzymatic reaction constants K(m) and k(cat). The acoustofluidic design involves a bubble anchored in a horseshoe structure which can be stimulated by a piezoelectric transducer to generate
K D Wittrup et al.
Cytometry, 9(4), 394-404 (1988-07-01)
A novel assay of single-cell exogenous beta-galactosidase activity in Saccharomyces cerevisiae has been developed. Intracellular fluorescence due to the hydrolysis of resorufin-beta-D-galactopyranoside attains a steady state between production of resorufin and its subsequent leakage from the cell. The cells are
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