生物来源
chicken egg white
质量水平
表单
lyophilized powder
浓度
≥50% protein (biuret)
UniProt登记号
储存温度
−20°C
基因信息
chicken ... RBP(396449)
一般描述
Riboflavin Binding Protein (RBP) comprises of 19 cysteines and corresponds to a molecular weight of 30 kDa. Egg white RBP is produced in the oviduct cells. Chicken egg white RBP contains an N-terminal ligand binding domain and a C-terminal phosphorylated motif.
Riboflavin binding protein (RBP) transports riboflavin through the bloodstream of the laying hen into the eggs, where the vitamin is essential for embryonic growth and development. RBP consists of a single polypeptide chain containing 9 disulfide bonds. It is a glycoprotein containing ~14% carbohydrate, with sugar moieties including galactose, mannose and glucosamine, and is phosphorylated.
应用
Riboflavin Binding Protein from chicken egg white may be used in fluorescence quenching experiments.
生化/生理作用
Riboflavin Binding Protein (RBP) is crucial for normal embryo development. RBP interacts with riboflavin and its analogs. The RBP-riboflavin complex is stabilized by interaction between the isoalloxazyne ring of riboflavin and the tyrosine 75 and tryptophan 156 of residues of RBP. RBP elicits sweet supressing functionality and may interact with the sweet taste receptor.
Riboflavin binding protein binds to flavokinase and regulates its activity.
制备说明
Supplied as the riboflavin-depleted apo-form.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物来源生物产品
此项目有
Riboflavin-binding protein exhibits selective sweet suppression toward protein sweeteners
Maehashi K, et al.
Chemical Senses, 32(2), 183-190 (2006)
Binding study of riboflavin-binding protein with riboflavin and its analogues by differential scanning calorimetry
Wasylewski M
Journal of Protein Chemistry, 19(6), 523-528 (2000)
Electrochemistry of riboflavin-binding protein and its interaction with riboflavin
Bartovsik M, et al.
Bioelectrochemistry, 76(1-2), 70-75 (2009)
Crystal structure of chicken riboflavin-binding protein
Monaco HL
The Embo Journal, 16(7), 1475-1483 (1997)
The effect of riboflavin binding protein (RPB) on flavokinase catalytic activity.
Slomczynska, M.
Comparative Biochemistry and Physiology, 87, 681-685 (1987)
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