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Merck
CN

R5405

Sigma-Aldrich

Anti-Rabex-5 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-RAP1, Anti-Rab5 GDP/GTP exchange factor (RABGEF1), Anti-Rabaptin-5-associated exchange factor for Rab5

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UNSPSC代码:
12352203
NACRES:
NA.41

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生物来源

rabbit

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

分子量

antigen ~58 kDa

种属反应性

mouse, rat, human

浓度

~1.0 mg/mL

技术

indirect immunofluorescence: 5-10 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin
western blot: 2-5 μg/mL using whole extracts of mouse brain and human U87 cells

UniProt登记号

Q9UJ41

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... RABGEF1(27342)
mouse ... Rabgef1(56715)
rat ... Rabgef1(360797)

一般描述

Anti-Rabex-5 (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to a sequence at N-terminal of human Rabex-5, with an added cysteine, conjugated to KLH. Rabex-5 contains multiple functional domains, including a vacuolar protein sorting-associated (VPS9) domain that possesses the GDP/GTP exchange factors (GEF) activity, a ubiquitin-binding A20-like zinc finger (ZnF) domain, and a coiled-coil domain.

应用

Anti-Rabex-5 (N-terminal) antibody may be used in immunoblotting and immunofluorescence.

生化/生理作用

Rabex-5 forms a tight complex with Rabaptin-5 that is essential for endocytic membrane fusion. The bound Rabaptin-5 to Rabex-5 increases the GEF activity of Rabex-5 and fastens the generation of the active GTP-bound form of membrane associated Rab5.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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分析证书(COA)

Lot/Batch Number
104M4828V
078K4805

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Functional synergy between Rab5 effector Rabaptin-5 and exchange factor Rabex-5 when physically associated in a complex
Lippe R, et al.
Molecular Biology of the Cell, 12(7), 2219-2228 (2001)
The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain
Mattera R, et al.
Test, 281(10), 6874-6883 (2006)
Kavitha Balaji et al.
Traffic (Copenhagen, Denmark), 15(11), 1206-1218 (2014-08-02)
Listeria monocytogenes is a food-borne pathogenic bacterium that invades intestinal epithelial cells through a phagocytic pathway that relies on the activation of host cell RAB5 GTPases. Listeria monocytogenes must subsequently inhibit RAB5, however, in order to escape lysosome-mediated destruction. Relatively
The RAB5-GEF Function of RIN1 Regulates Multiple Steps During Listeria monocytogenes Infection.
Kavitha Balaji et al.
Traffic (Copenhagen, Denmark), 16(7), 796-796 (2015-06-09)
David Millrine et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(38), 10625-10630 (2016-09-08)
Immunomodulatory drugs (IMiDs) are a family of compounds derived from thalidomide. Binding of the IMiD molecule to the Lon protease Cereblon initiates the degradation of substrates via the ubiquitin proteasome pathway. Here, we show that Cereblon forms a complex with

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