一般描述
转移RNA（tRNA）是通过苯酚-氯仿萃取和乙醇沉淀从baker′s酵母中分离得到。tRNA是一类大约具有80个核苷酸长度的RNA分子，具有苜蓿叶形的二级结构和三级L形结构。
应用
适合用作核酸纯化和沉淀的载体。
从bakers′酵母（酿酒酵母）中分离得到的核糖核酸用于：
从bakers′酵母（酿酒酵母）中分离得到的核糖核酸用于：
- 载体溶液中，作为对照的一部分用于逆转录酶–定量聚合酶反应（RT-qPCR）分析
- 在单标记原位杂交中，作为预杂交（4）和杂交缓冲液的组成部分
- 作为增强从少量细胞中回收RNA的载体
生化/生理作用
转移RNA（tRNA）在翻译过程中起重要作用。它负责向核糖体中添加氨基酸以形成肽链。tRNA包含一个用于mRNA碱基配对的反密码子区域和两个附着区域：用于氨基酸结合和tRNA合成酶识别。核糖体RNA能够促进tRNA沿mRNA的移动。
制备说明
tRNA 溶于 1 mM EDTA 的 10 mM Tris HCl (pH 7.4) 溶液中。
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Marilyn C Olson et al.
Methods in molecular biology (Clifton, N.J.), 258, 53-69 (2004-02-19)
The Invader assay is a homogeneous, isothermal, signal amplification system for the quantitative detection of nucleic acids. The assay can directly detect either DNA or RNA without target amplification or reverse transcription. It is based on the ability of Cleavase
Oliver Mühlemann et al.
Methods in molecular medicine, 131, 33-46 (2007-07-28)
Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro
Eric A Nalefski et al.
iScience, 24(9), 102996-102996 (2021-09-11)
Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by activating RNA-programmable RNases and DNases. Cas13a and Cas12a enzymes bound to CRISPR RNA (crRNA) recognize specific nucleic acid targets, initiating cleavage of the targets as well as non-target (trans)
Naming 'junk': human non-protein coding RNA (ncRNA) gene nomenclature
Wright MW and Bruford EA
Human Genomics, 5(2), 90-90 (2011)
A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability
Redshaw N, et al.
Biotechniques, 54(3), 155-164 (2013)
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