R5886
RPMI-1640 培养基
HEPES modification, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
别名:
Roswell Park Memorial Institute 1640 medium
产品名称
RPMI-1640 培养基, HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
RPMI-1640培养基已用于：
- 培养人淋巴母细胞系细胞
- 前列腺癌细胞
- 人血单核细胞
General description
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
该RPMI-1640培养基补充了HEPES。 HEPES是一种Good′s生理缓冲液，有助于提供比碳酸氢盐缓冲液更稳定的培养基pH。
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源培养基
此项目有
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