产品名称
Anti-ROCK-1 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 160 kDa
species reactivity
human, rat, mouse
technique(s)
immunoprecipitation (IP): 10-20 μL using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line
microarray: suitable
western blot: 1:1,000-1:2,000 using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ROCK1(6093)
mouse ... Rock1(19877)
rat ... Rock1(81762)
Application
Anti-ROCK-1 antibody produced in rabbit has been used in western blotting and immunocytochemistry.
Biochem/physiol Actions
Rho-associated kinases (ROCK or Rho-kinase) is implicated in Rho-mediated actin reorganization such as formation of stress fibres and focal adhesions and smooth muscle contraction. Rho-kinases lead to an increase of myosin light chain (MLC) phosphorylation and thereby induce actomyosin based contractility. ROCK accumulates at the cleavage furrow during cytokinesis. In addition, a dominant negative form of ROCK increases multinuclei in mammalian cells. ROCK directly phosphorylates and activates LIM-kinase (LIMK), resulting in downstream phosphorylation and inaction of the actin depolymerizing factor or cofilin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Rho-associated coiled-coil protein kinase 1 or ROCK-1 is a serine/threonine kinase that regulates actin assembly, cell movement and contraction. This kinase activates the JNK signaling pathway, modulates stress granule formation and regulates apoptosis in cardiac myocytes and stress granules .
Immunogen
synthetic peptide corresponding to amino acids 1336-1354 located at the C-terminus of human ROCK-1, conjugated to KLH. This sequence is identical in mouse and rat ROCK-1.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions
Katoh K, et al.
Journal of the Royal Society, Interface / the Royal Society, 8(56), 305-305 (2011)
Rho plays a key role in TGF-beta1-induced proliferation and cytoskeleton rearrangement of human periodontal ligament cells
Wang L, et al.
Archives of Oral Biology, 59(2), 149-157 (2014)
Jiang Chang et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(39), 14495-14500 (2006-09-20)
Rho-associated coiled-coil protein kinase 1 (ROCK-1) is a direct cleavage substrate of activated caspase-3, which is associated with heart failure. In the course of human heart failure, we found marked cleavage of ROCK-1 resulting in a 130-kDa subspecies, which was
Pat P Ongusaha et al.
Science signaling, 1(47), ra14-ra14 (2008-11-28)
Although apoptosis triggered by ultraviolet B (UVB)-mediated activation of the c-Jun N-terminal kinase (JNK) pathway is mediated by both intrinsic and extrinsic pathways, the mechanism of initiation of JNK activation remains obscure. Here, we report the characterization of the JNK-interacting
Marc Jacobs et al.
The Journal of biological chemistry, 281(1), 260-268 (2005-10-27)
ROCK or Rho-associated kinase, a serine/threonine kinase, is an effector of Rho-dependent signaling and is involved in actin-cytoskeleton assembly and cell motility and contraction. The ROCK protein consists of several domains: an N-terminal region, a kinase catalytic domain, a coiled-coil
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