R7388
RPMI-1640 培养基
With HEPES, with ʟ-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
别名:
Roswell Park Memorial Institute 1640 medium
产品名称
RPMI-1640 培养基, Modified, with 20 mM HEPES and L-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
RPMI-1640培养基已用于培养：
- 人永生角化细胞、体外转染试验
- 细胞系和人T细胞母细胞，监控自噬流量
- 小猪外周血单核细胞(PBMC)
General description
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
RPMI-1640培养基配方基于RPMI-1630系列培养基，利用碳酸氢盐缓冲系统和氨基酸和维生素数量的改变。用于人正常白细胞和肿瘤白细胞的培养。RPMI-1640还支持各种类型的细胞培养物的生长，如72小时植物血凝素(PHA)刺激试验中的新鲜人淋巴细胞。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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