grade
Molecular Biology
Quality Level
form
liquid
usage
0.1 mL sufficient for 100 RT-PCR reactions
concentration
50 μM in H2O
shipped in
wet ice
storage temp.
−20°C
General description
随机引物是由9个脱氧核苷酸组成的随机序列(9-mers)，可作为有或无poly(A)尾的mRNA的逆转录引物。随机引物也可作为通用的非特异性引物。
Application
随机九聚体适用于：
- 第一链 cDNA 合成
- 制备 cDNA 库（使用寡聚（dT）引物
- 制备用于杂交的已标记的DNA探针
Features and Benefits
- 当制备 cDNA 库或处理特异性引物不适合的情况时，可以将随机九聚体与锚定寡聚 (dT)23 引物（货号 O4387）组合。
- 在防止序列信息不完整或缺失，或特异性引物无效的情况下，随机九聚体为 cDNA 库提供了解决方案。
- 随机九聚体′ 在较高温度（高达 65°C）下的引发能力会降低，请确保它们不会干扰转录后的聚合酶链式反应 (PCR)。
- 随机九聚体也可用于制备杂交研究的标记DNA探针。随机九聚体可替代特异性逆转录(RT)引物，用于第一链合成、cDNA库构建和其他应用。
Other Notes
仅供实验室使用。不可用于药物、家庭或其他用途。
随机引物以50 uM 水溶液形式提供。
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
P Goelet et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(19), 5818-5822 (1982-10-01)
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 10-10 (1989)
Hsiao-Wen Lin et al.
Journal of neurochemistry, 111(3), 808-818 (2009-08-29)
Interleukin-6 (IL-6) is produced by neurons, astrocytes, and microglia, and elevated levels of IL-6 within the CNS have been documented in multiple neurological disorders including Alzheimer's disease, stroke, epilepsy, attention deficit disorder, cerebral palsy, and multiple sclerosis. Here, we sought
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R7647-100UL | 04061837898952 |