R7755
RPMI-1640 培养基
AutoMod™, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture
别名:
Roswell Park Memorial Institute 1640 medium
产品名称
RPMI-1640 培养基, AutoMod™, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
Other Notes
经改良可用于高压灭菌
不含L-谷氨酰胺和碳酸氢钠。
不含L-谷氨酰胺和碳酸氢钠。
Preparation Note
按照每升培养基10.3克粉末配制。
补充0.3 L-谷氨酰胺，2.0 g/L碳酸氢钠。
Legal Information
Auto-Mod is a trademark of Sigma-Aldrich Co. LLC
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源培养基
此项目有
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实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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