grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
3,000-10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
HpaI is a restriction endonuclease that is used to cleave DNA at the recognition site 5′-GTT/AAC-3′, generating fragments with blunt ends.
Biochem/physiol Actions
Recognition sequence: 5′-GTT/AAC-3′
Ligation and recutting results: After 2-10-fold Hpa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Not completely inactivated at 65 °C for 15 minutes.
Ligation and recutting results: After 2-10-fold Hpa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Not completely inactivated at 65 °C for 15 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.5 mM EDTA, 5 mM 2-mercaptoethanol, 0.01% polydocanol, 50% glycerol (v/v) at 4°C
Other Notes
Supplied with 10x Restriction Enzyme Buffer SA (B7531).
存储类别
12 - Non Combustible Liquids
怎么回事？
WGK 1
闪点 (F)
Not applicable
闪点 (°C)
Not applicable