grade
Molecular Biology
Quality Level
form
buffered aqueous solution
concentration
9-11 mg/mL
foreign activity
DNase, Nickase, none detected
shipped in
dry ice
storage temp.
−20°C
General description
转运RNA(tRNA)是一条RNA核苷酸短链，呈L型。
转移 RNA (tRNA) 是从面包酵母中分离得到的。
Application
从面包酵母(S. cerevisiae)转移的核糖核酸适合用作核酸纯化和沉淀中的载体。
来自面包酵母 (S. cerevisiae)的转运核糖核酸可用于裸卵(denuded oocyte)mRNA沉淀和逆转录。还用于在鼻咽癌(NPC)细胞RNA pull-down实验中封闭磁珠。
Biochem/physiol Actions
转运RNA(tRNA)作为衔接分子将mRNA序列中的三核苷酸序列（密码子）翻译为对应的氨基酸序列。通过连接配对的氨基酸和核酸来确定遗传密码。tRNA参与病毒复制、细菌细胞壁生物合成、细胞应激反应和动物行为控制。tRNA分子异常与多种人类疾病有关。
Preparation Note
tRNA 溶于 1 mM EDTA 的 10 mM Tris HCl (pH 7.4) 溶液中。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Patrick Lonergan et al.
Molecular reproduction and development, 66(3), 297-305 (2003-09-23)
In the cyclic cow, final maturation of the ovulatory follicle is initiated by the preovulatory luteinizing hormone (LH) surge. During the subsequent 24 hr period, the oocyte nucleus undergoes meiotic progression to metaphase II and several changes in cytoplasmic organization
Encyclopedia of Agriculture and Food Systems (2015)
Wolf D Hirschmann et al.
Nucleic acids research, 42(6), 4043-4055 (2014-01-22)
The budding yeast multi-K homology domain RNA-binding protein Scp160p binds to >1000 messenger RNAs (mRNAs) and polyribosomes, and its mammalian homolog vigilin binds transfer RNAs (tRNAs) and translation elongation factor EF1alpha. Despite its implication in translation, studies on Scp160p's molecular
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R8508-1ML | 04061838107183 |
| R8508-5X1ML | 04061838099785 |