R8654
Anti-RAD17 (N-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
别名:
Anti-S.cerevisiae homolog of RAD24, Anti-hRad17
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 75 kDa
species reactivity
mouse, rat, human
packaging
antibody small pack of 25 μL
technique(s)
immunoprecipitation (IP): suitable, western blot: 1:500-1:1,000 using HeLa cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAD17(5884)
mouse ... Rad17(19356)
rat ... Rad17(310034)
General description
Cell cycle checkpoint protein RAD17 is mapped to human chromosome 5q13.2 . It is also called s a replication factor C (RFC)-like DNA damage sensor protein. Eight alternative spliced transcript variants of the gene, which encode four distinct proteins, have been reported.
Immunogen
synthetic peptide corresponding to amino acids 2-15 of human RAD17 (isoform 1), conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide differs from that of isoform 2 by two amino acids.
Application
Anti-RAD17 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
RAD17 has been reported in breast, non-small cell lung carcinoma and breast cancer. RAD17 is a critical event during checkpoint signaling in DNA-damaged cells. Treatment of human cells with genotoxic agents induced ATM/ATR dependent phosphorylation of RAD17 at Ser635 and Ser645 , suggesting that phosphorylation of RAD17 is a critical event during checkpoint signaling in DNA-damaged cells.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
Human Rad17 is phosphorylated upon DNA damage and also overexpressed in primary non-small cell lung cancer tissues
Wang X, et al.
Cancer research, 61(20), 7417-7421 (2001)
Basal-like Breast cancer DNA copy number losses identify genes involved in genomic instability, response to therapy, and patient survival
Weigman VJ, et al.
Breast Cancer Research and Treatment, 133(3), 865-880 (2012)
Yasunori Fukumoto et al.
The Journal of biological chemistry, 297(2), 100831-100831 (2021-06-27)
The ATR pathway is one of the major DNA damage checkpoints, and Rad17 is a DNA-binding protein that is phosphorylated upon DNA damage by ATR kinase. Rad17 recruits the 9-1-1 complex that mediates the checkpoint activation, and proteasomal degradation of
Eun Kyeong Oh et al.
International journal of oncology, 41(6), 2038-2046 (2012-10-02)
Host genomic alterations in addition to human papillomavirus (HPV) are needed for cervical precursor lesions to progress to invasive cancer because only a small
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