产品名称
Visfatin EIA 试剂盒, for serum, plasma, culture supernatant and cell lysates
species reactivity
rat, human, mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
enzyme immunoassay: suitable
input
sample type serum
sample type culture supernatant(s)
sample type cell lysate
sample type plasma
assay range
inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 0.778 ng/mL
standard curve range: 0.1-1000 ng/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... NAMPT(10135)
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Visfatin EIA Kit has been used to measure the concentration of visfatin in blood samples.
Visfatin EIA Kit has been used to measure the concentration of visfatin in blood samples.
Biochem/physiol Actions
Visfatin has a role in mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis. Studies have shown that mice deficient in visfatin in the forebrain excitatory neurons show hyperactivity, diminished anxiety-like behaviors and impaired learning and memory. It has also been shown that visfatin stimulates glucose uptake and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation in mouse C2C12 skeletal muscle cells. Visfatin regulates inflammation and insulin sensitivity.
General description
The Visfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Visfatin peptide based on the principle of Competitive Enzyme Immunoassay.
Visfatin, also known as nicotinamide phosphoribosyltransferase (Nampt)/ pre-B Cell colony enhancing factor is encoded by the gene mapped to human chromosome 7q22.3. The gene contains 11 exons encompassing 34.7kb. The encoded protein is an adipocytokine secreted by visceral fat.
Visfatin, also known as nicotinamide phosphoribosyltransferase (Nampt)/ pre-B Cell colony enhancing factor is encoded by the gene mapped to human chromosome 7q22.3. The gene contains 11 exons encompassing 34.7kb. The encoded protein is an adipocytokine secreted by visceral fat.
Immunogen
Visfatin, synthetic peptide
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
存储类别
8A - Combustible corrosive hazardous materials
法规信息
低风险生物材料
常规特殊物品
此项目有
A visfatin promoter polymorphism is associated with low?grade inflammation and type 2 diabetes.
Zhang Y-Y, et al.
Obesity (Silver Spring, Md.), 14(12), 2119-2126 (2006)
Matthaios G Pitoulias et al.
Cytokine, 91, 140-144 (2017-01-11)
Visfatin is an adipokine molecule acting as an essential coenzyme in multiple cellular redox reactions. The increased serum levels of Visfatin have been correlated with metabolic syndrome and endothelial homeostasis. In this study we investigate the possible relationship of Visfatin
Effects of Diet and Exercise-Induced Weight Loss in Sedentary Obese Women on Inflammatory Markers, Resistin, and Visfatin.
Khanna D, et al.
Obesity and Nutrition of J, 102 (2017)
Cahit Birdir et al.
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, 30(6), 629-634 (2016-04-29)
Investigating potential value of maternal serum Visfatin, sFlt-1, PlGF, AFP, PAPP-A levels at first trimester for prediction of small for gestational age (SGA) at birth. Measurements were performed in 20 SGA and 65 control cases. Logistic regression analysis adjusted for
Ashleigh M Philp et al.
International journal of molecular sciences, 22(13) (2021-07-03)
Obesity increases the risk of hip osteoarthritis (OA). Recent studies have shown that adipokine extracellular nicotinamide phosphoribosyltransferase (eNAMPT or visfatin) induces the production of IL-6 and matrix metalloproteases (MMPs) in chondrocytes, suggesting it may promote articular cartilage degradation. However, neither
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Instructions
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