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安全信息

RAB0610

Sigma-Aldrich

人 APOB/载脂蛋白 B-100 ELISA 试剂盒

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别名:
Apo B-100, Apolipoprotein B-100
NACRES:
NA.32

种属反应性

human

包装

kit of 96 wells (12 strips x 8 wells)

技术

ELISA: suitable

输入

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2.5 ng/mL
standard curve range: 2.458-600 ng/mL

检测方法

colorimetric

运输

wet ice

储存温度

−20°C

基因信息

human ... APOB(338)

一般描述

The ApoB ELISA kit provides for the Quantitative measurement of ApoB-100 in Cell Culture Supernatants, Plasma and Serum.
The APOB (apolipoprotein B) gene is mapped to human chromosome 2p24.1. Apob protein is an amphipathic protein that exists as α- helices and β-sheets. Apob is present in the major lipoproteins : chylomicrons, low density lipoproteins and very low density lipoprotein.

应用

请参考Protocol了解详情。

生化/生理作用

Apob (apolipoprotein B) functions in the low-density lipoprotein cholesterol segregation. Increased APOB gene expression is observed in non-alcoholic fatty liver disease and contributes to its pathogenesis. Reduced Apob protein is indicated in familial hypobetalipoproteinemia.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)化学品安全说明书

  • RABSTOP3ELISA Stop Solution (Item I)化学品安全说明书

  • RABWASH420X Wash Buffer (Item B)化学品安全说明书

象形图

Corrosion

警示用语:

Warning

危险声明

预防措施声明

危险分类

Met. Corr. 1

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Lipoproteomics: using mass spectrometry-based proteomics to explore the assembly, structure, and function of lipoproteins.
Hoofnagle A N and Heinecke J W
Journal of Lipid Research, 50(10), 1967-1975 (2009)
Serum apoB levels independently predict the development of non-alcoholic fatty liver disease: A 7-year prospective study.
Wang J, et al.
Liver International : Official Journal of the International Association For the Study of the Liver, 37(8), 1202-1208 (2017)
Identification of novel APOB mutations by targeted next-generation sequencing for the molecular diagnosis of familial hypobetalipoproteinemia.
Rimbert A, et al.
Atherosclerosis, 250, 52-56 (2016)
Alejandro Escobedo et al.
Nutrients, 13(11) (2021-11-28)
Snack alternatives based on common beans (Phaseolus vulgaris L.) have been developed to promote pulse consumption. The purpose of this study was to evaluate the chemical composition, sensory acceptance and the effect of common bean baked snack (CBBS) consumption on
Forty-three loci associated with plasma lipoprotein size, concentration, and cholesterol content in genome-wide analysis.
Chasman DI, et al.
PLoS Genetics, 5(11), e1000730-e1000730 (2009)

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