产品名称
ReadyScript® cDNA 合成混合物, Complete reagent for first strand cDNA synthesis for RT-qPCR
usage
sufficient for 100 reactions
sufficient for 25 reactions
sufficient for 500 reactions
feature
dNTPs included
hotstart: no
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
dry ice
storage temp.
−20°C
Application
Features and Benefits
- 以特定比例包含随机和寡聚 (dT) 引物，以确保 cDNA 产品中所有转录本序列的最佳表达。
- 高重复性预混液消除了其他需要成分混合的试剂盒固有的可变性
- RNase H(+) Moloney 鼠白血病病毒 (MMLV) 逆转录酶确保残余 RNA 不干扰 PCR 扩增
- 可在广泛的模板起始量范围下逆转录罕见和丰富基因
- 方便的 5X 预混液配方，快速简便的反应设置；只需添加 RNA 模板
- 方便的 5X 预混液配方，快速简便的反应设置；只需添加 RNA 模板
- 包括随机因子和oligodT引物的专有混合，以及Moloney鼠白血病病毒(MMLV)逆转录酶的RNAse H+衍生物
- 可在40分钟内无偏差、准确地将RNA转化为cDNA
- 在一次20μL的反应中可以使用1μg的RNA；反应也可以按比例放大
- 高度稳定；-20°C下最长保存1年；4°C下最长保存一个月
General description
Other Notes
Legal Information
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
商品
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
实验方案
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。
相关内容
PCR是分子生物学的核心技术，功能强大。可在体外高效快速地对不同来源的特定DNA或RNA序列进行酶扩增。
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
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