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RECOMT

凝血酶 CleanCleave 试剂盒

别名:

Thrombin Cleavage Kit

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关于此项目

NACRES:
NA.56
UNSPSC Code:
12352200
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form

suspension

Quality Level

mol wt

37 kDa

technique(s)

protein purification: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

凝血酶 CleanCleave 试剂盒包含通过固定牛凝血酶产生的 50% 凝血酶 - 琼脂糖悬浮液,用于切割重组融合蛋白

Application

用于从含有凝血酶识别序列的重组融合蛋白切割标签。凝血酶 - 琼脂糖与在各种类型的表达系统中表达的重组蛋白相容。

Features and Benefits

  • 在短短 2 小时内快速、有效地裂解
  • 凝血酶与琼脂糖共价结合,易于除去。
  • 在 4°C 到 37°C 的温度范围内,以及在广泛的 pH 和离子强度范围内,强裂解反应是有效的。
  • 即使在 0.1%Triton,1M 尿素或 5mM EDTA 存在下,也能裂解标签
  • 凝血酶 - 琼脂糖是可重复使用的

Preparation Note

200 μl 50% 的凝血酶 - 琼脂糖浆液裂解 >85% 的 1mg 融合蛋白。

Legal Information

CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

仅试剂盒组分

产品编号
说明

  • Thrombin Cleavage Buffer, 10× 10 mL

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品

此项目有


历史批次信息供参考:

分析证书(COA)

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Claudia H Huichalaf et al.
Human molecular genetics, 28(12), 2014-2029 (2019-02-13)
An early hallmark of Alzheimer's disease is the accumulation of amyloid-β (Aβ), inspiring numerous therapeutic strategies targeting this peptide. An alternative approach is to destabilize the amyloid beta precursor protein (APP) from which Aβ is derived. We interrogated innate pathways
Linda J Urbanski et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 1834-1839 (2020-09-26)
This paper presents the production and kinetic and inhibitory characterisation of β-carbonic anhydrase from the opportunistic bacterium Staphylococcus aureus (SauBCA). From the eight different carbonic anhydrase (CA) families known to date, humans have only the α-form, whereas many clinically relevant
Robin Levy et al.
Biomolecules, 9(3) (2019-03-06)
The disordered p53 transactivation domain (p53TAD) contains specific levels of transient helical secondary structure that are necessary for its binding to the negative regulators, mouse double minute 2 (Mdm2) and MdmX. The interactions of p53 with Mdm2 and MdmX are
Matthew R Reynolds et al.
Biochemistry, 44(5), 1690-1700 (2005-02-03)
Alzheimer's disease (AD) is a progressive amnestic disorder typified by the pathological misfolding and deposition of the microtubule-associated tau protein into neurofibrillary tangles (NFTs). While numerous post-translational modifications influence NFT formation, the molecular mechanisms responsible for tau aggregation remain enigmatic.
Andrea Angeli et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 758-763 (2021-03-16)
We report the first activation study of the β-class carbonic anhydrase (CA, EC 4.2.1.1) encoded in the genome of the protozoan pathogen Trichomonas vaginalis, TvaCA1. Among 24 amino acid and amine activators investigated, derivatives incorporating a second carboxylic moiety, such

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