form
suspension
Quality Level
mol wt
37 kDa
technique(s)
protein purification: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
凝血酶 CleanCleave 试剂盒包含通过固定牛凝血酶产生的 50％ 凝血酶 - 琼脂糖悬浮液，用于切割重组融合蛋白
Application
用于从含有凝血酶识别序列的重组融合蛋白切割标签。凝血酶 - 琼脂糖与在各种类型的表达系统中表达的重组蛋白相容。
Features and Benefits
- 在短短 2 小时内快速、有效地裂解
- 凝血酶与琼脂糖共价结合，易于除去。
- 在 4°C 到 37°C 的温度范围内，以及在广泛的 pH 和离子强度范围内，强裂解反应是有效的。
- 即使在 0.1％Triton™，1M 尿素或 5mM EDTA 存在下，也能裂解标签
- 凝血酶 - 琼脂糖是可重复使用的
Preparation Note
200 μl 50％ 的凝血酶 - 琼脂糖浆液裂解 >85% 的 1mg 融合蛋白。
Legal Information
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
仅试剂盒组分
产品编号
说明
- Thrombin Cleavage Buffer, 10× 10 mL
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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An early hallmark of Alzheimer's disease is the accumulation of amyloid-β (Aβ), inspiring numerous therapeutic strategies targeting this peptide. An alternative approach is to destabilize the amyloid beta precursor protein (APP) from which Aβ is derived. We interrogated innate pathways
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实验方案
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