form
suspension
mol wt
37 kDa
technique(s)
protein purification: suitable
shipped in
wet ice
storage temp.
2-8°C
Quality Level
General description
凝血酶 CleanCleave 试剂盒包含通过固定牛凝血酶产生的 50％ 凝血酶 - 琼脂糖悬浮液，用于切割重组融合蛋白
Preparation Note
200 μl 50％ 的凝血酶 - 琼脂糖浆液裂解 >85% 的 1mg 融合蛋白。
Legal Information
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Application
用于从含有凝血酶识别序列的重组融合蛋白切割标签。凝血酶 - 琼脂糖与在各种类型的表达系统中表达的重组蛋白相容。
Features and Benefits
- 在短短 2 小时内快速、有效地裂解
- 凝血酶与琼脂糖共价结合，易于除去。
- 在 4°C 到 37°C 的温度范围内，以及在广泛的 pH 和离子强度范围内，强裂解反应是有效的。
- 即使在 0.1％Triton™，1M 尿素或 5mM EDTA 存在下，也能裂解标签
- 凝血酶 - 琼脂糖是可重复使用的
仅试剂盒组分
产品编号
说明
- Thrombin Cleavage Buffer, 10× 10 mL
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Andrea Angeli et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 758-763 (2021-03-16)
We report the first activation study of the β-class carbonic anhydrase (CA, EC 4.2.1.1) encoded in the genome of the protozoan pathogen Trichomonas vaginalis, TvaCA1. Among 24 amino acid and amine activators investigated, derivatives incorporating a second carboxylic moiety, such
Carlos Alvarado et al.
Molecules (Basel, Switzerland), 24(18) (2019-09-22)
The Focal Adhesion Targeting (FAT) domain of Focal Adhesion Kinase (FAK) is a promising drug target since FAK is overexpressed in many malignancies and promotes cancer cell metastasis. The FAT domain serves as a scaffolding protein, and its interaction with
Matthew R Reynolds et al.
Biochemistry, 44(5), 1690-1700 (2005-02-03)
Alzheimer's disease (AD) is a progressive amnestic disorder typified by the pathological misfolding and deposition of the microtubule-associated tau protein into neurofibrillary tangles (NFTs). While numerous post-translational modifications influence NFT formation, the molecular mechanisms responsible for tau aggregation remain enigmatic.
Takeshi Uchida et al.
The Journal of biological chemistry, 279(50), 51981-51988 (2004-10-07)
The heme chaperone CcmE is a novel protein that binds heme covalently via a histidine residue as part of its essential function in the process of cytochrome c biogenesis in many bacteria as well as plant mitochondria. In the continued
Manish Gupta et al.
Frontiers in microbiology, 7, 886-886 (2016-07-06)
Mycobacterium tuberculosis H37Rv escapes host-generated stresses by entering a dormant persistent state. Activation of toxin-antitoxin modules is one of the mechanisms known to trigger such a state with low metabolic activity. M. tuberculosis harbors a large number of TA systems
商品
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
实验方案
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持