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Merck
CN

RT7POL

Sigma-Aldrich

T7 RNA Polymerase

≥50,000 units/mL, aqueous solution

别名:

bacteriophage T7 RNA polymerase, T7 RNAP, e coli T7 RNA polymerase, in vitro mRNA polymerase, recombinant T7

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UNSPSC代码:
12352204

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生物来源

bacteriophage T7

质量水平

200

重组

expressed in E. coli

等级

Molecular Biology

方案

≥95% (SDS-PAGE)

表单

aqueous solution (containing 50% glycerol)
liquid

比活

≥50000 units/mL

保质期

2 yr at -20 °C

分子量

11.4 kDa

储存条件

OK to freeze

浓度

≥50000 units/mL

颜色

colorless

最佳pH

7.7 (37 °C)

pH值（酸碱度）

7.9 (25 °C)

适用性

suitable for (In-vitro RNA transcription)

应用

research use

异质活性

DNAse, none detected

RNAse, none detected

Nickase, none detected

运输

dry ice

储存温度

-10 to -25°C

一般描述

T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5′ to 3′ direction. The T7 polymerase also requires a double stranded DNA template and Mg2+ ion as cofactor for the synthesis of RNA. It has a very low error rate.

应用

This product is a recombinant protein expressed in E. coli and induced by IPTG. The MW of T7 RNA Polymerase is 98.9 kDa and theoretical pI of ~6.8.

特点和优势

T7 polymerase is extremely promoter-specific, transcribes only DNA downstream of a T7 promoter, and can be used for in vitro mRNA synthesis. Quality tests include:
  • Nickase activty: None detected in a 50 µl reaction containing 250 ng of supercoiled pUC19 DNA and at least 30 units of T7 RNA Polymerase incubated for 4 hours at 37°C.
  • DNase Activity: None detected in a 50 µl reaction containing 250 ng linear pUC19 DNA and at least 30 units of T7 RNA Polymerase incubated for 16 hours at 37°C.
  • RNase Activity: None detected in a 50 µl reaction containing 1.25-2.5 ng tRNA and at least 30 units of T7 RNA Polymerase is incubated for 4h at 37°C.

外形

The product is supplied as an aqueous solution containing 50mM Tris-HCl, 100mM NaCl, 20mM β-ME, 1mM EDTA, 0.02% (w/v) Tween 20 and 50% glycerol, pH 7.9 at 25 °C.

其他说明

1 Unit of T7 RNA polymerase is defined as the amount of enzyme that synthesizes 1ug of mRNA per hour in a 30-minute reaction containing 25μg/ml linearized plasmid, 3mM of each NTP and 18mM MgCl2, at 37°C, pH 7.7

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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分析证书(COA)

Lot/Batch Number
0000404475
0000404474
0000404474
0000404475

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Promoter specificity determinants of T7 RNA polymerase.
Rong M, et al.
Proceedings of the National Academy of Sciences of the USA, 95, 515-519 (1998)

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Product Information Sheet -rt7pol

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