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Merck
CN

S0942

Sigma-Aldrich

磷酸酶底物

chromogenic, tablet (round)

别名:

4-硝基苯磷酸盐 二钠盐 六水合物, pNPP 二钠盐 六水合物

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关于此项目

线性分子式:
O2NC6H4OP(O)(ONa)2 · 6H2O
化学文摘社编号:
分子量:
371.14
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.54
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产品名称

磷酸酶底物, 5 mg tablets

质量水平

表单

tablet (round)

储存温度

−20°C

SMILES字符串

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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相关类别

一般描述

酸性和碱性磷酸酶测定用底物

应用

用于碱性和酸性磷酸酶ELISA法的发色底物。产生可溶性终产物,黄色,分光光度法在405nm处读取。pNPP反应能被3N NAOH终止,在405nm处读取。

其他说明

Substrate for the determination of acid and alkaline phosphatases.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Thomas F Rogers et al.
Science (New York, N.Y.), 369(6506), 956-963 (2020-06-17)
Countermeasures to prevent and treat coronavirus disease 2019 (COVID-19) are a global health priority. We enrolled a cohort of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-recovered participants, developed neutralization assays to investigate antibody responses, adapted our high-throughput antibody generation pipeline
Yeon-Suk Yang et al.
Molecular therapy. Methods & clinical development, 17, 922-935 (2020-05-15)
Improper activity of bone-resorbing osteoclasts results in low bone density and deterioration of bone structure, which increase the risk of fractures. Anti-resorptive therapies targeting osteoclasts have proven effective in preserving bone mass, but these therapeutic agents lead to defective new
Lijun Wang et al.
Nature communications, 11(1), 282-282 (2020-01-17)
Wolff's law and the Utah Paradigm of skeletal physiology state that bone architecture adapts to mechanical loads. These models predict the existence of a mechanostat that links strain induced by mechanical forces to skeletal remodeling. However, how the mechanostat influences
Christina Papista et al.
Kidney international, 88(2), 276-285 (2015-03-26)
IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the
Rasmus Iversen et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(48), 17146-17151 (2014-11-19)
The multifunctional enzyme transglutaminase 2 (TG2) is the target of autoantibodies in the gluten-sensitive enteropathy celiac disease. In addition, the enzyme is responsible for deamidation of gluten peptides, which are subsequently targeted by T cells. To understand the regulation of

商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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