S1073
Anti-STUB1/CHIP antibody, mouse monoclonal
clone ST21.55, purified from hybridoma cell culture
别名:
Anti-C terminus of HSC70-interacting protein (CHIP), Anti-HSPABP2, Anti-Heat shock protein A binding protein 2 (c-terminal), Anti-NY-CO-7, Anti-SDCCAG7, Anti-STIP1 homologous and box-containing protein 1 (STUB1), Anti-Serologically defined colon cancer antigen 7, Anti-UBOX1
登录 查看组织和合同定价。
选择尺寸
关于此项目
Conjugate:
unconjugated
Clone:
ST21.55, monoclonal
Application:
ELISA (i), WB
Citations:
11
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
ST21.55, monoclonal
form
buffered aqueous solution
mol wt
antigen ~35 kDa
species reactivity
rat, human, bovine
concentration
~1.0 mg/mL
technique(s)
indirect ELISA: suitable, western blot: 2.5-5 μg/mL using HeLa total cell extract.
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
bovine ... STUB1(504565)
human ... STUB1(10273)
mouse ... Stub1(56424)
rat ... Stub1(287155)
General description
Monoclonal Anti-STUB1/CHIP (mouse IgG2b isotype) is derived from the hybridoma ST21.55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 30-45 of human STUB1/CHIP. STUB1/CHIP (carboxyl terminus of Hsc70-interacting protein) consists of three functional domains: a tetratricopeptide repeat (TRP) at the amino terminus, a U- box domain at the C-terminus, and a highly charged region separating the two.
STUB1 (STIP1 homology and U-box containing protein 1) or CHIP (carboxyl terminus of Hsc70- interacting protein) is an E3 ubiquitin ligase, (2) that is located on human chromosome 16p13.3.
Immunogen
synthetic peptide corresponding to amino acids 30-45 of human STUB1/CHIP.
Application
Anti-STUB1/CHIP antibody, mouse monoclonal has been used in immunoblotting and enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
In lens epithelial cells, knocking down CHIP (carboxyl terminus of Hsc70- interacting protein) decreased the reaction of heat shock and potential of protein quality control. It plays a major role in neurodegeneration and also controls autophagic flux. It can deactivate NF-κB signaling and damage the capability of migration and invasion in gastric cancer cells.
STUB1/CHIP (carboxyl terminus of Hsc70-interacting protein) is such a cofactor, which interacts, among others, with Hsc70 and acts as a U-box-dependent E3 ubiquitin ligase. STUB1/CHIP can also act as a direct chaperone of p53, both under physiological and stress conditions.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
CHIP Knockdown Reduced Heat Shock Response and Protein Quality Control Capacity in Lens Epithelial Cells
Zhang W, et al.
Current Molecular Medicine, 15(7), 652-662 (2015)
CHIP (carboxyl terminus of Hsc70-interacting protein) promotes basal and geldanamycin-induced degradation of estrogen receptor-alpha
Fan M, et al.
Molecular Endocrinology, 19(12), 2901-2914 (2005)
Ataxia and hypogonadism caused by the loss of ubiquitin ligase activity of the U box protein CHIP
Shi CH, et al.
Human Molecular Genetics, 23(4), 1013-1024 (2013)
Regulation of autophagic flux by CHIP
Guo D, et al.
Neuroscience Bulletin, 31(4), 469-479 (2015)
F Bertucci et al.
Oncogene, 27(40), 5359-5372 (2008-05-21)
Invasive ductal carcinomas (IDCs) and invasive lobular carcinomas (ILCs) are the two major pathological types of breast cancer. Epidemiological and histoclinical data suggest biological differences, but little is known about the molecular alterations involved in ILCs. We undertook a comparative
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持