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S2628

N-琥珀酰- L -苯丙氨酸- p -硝基苯胺

Name: N-琥珀酰-<SC> L </SC>-苯丙氨酸-<I> p </I>-硝基苯胺
Brand: SIGMA

protease substrate, ≥98% (TLC), powder

别名:

Suc-Phe-pNA

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关于此项目

经验公式（希尔记法）:

C₁₉H₁₉N₃O₆

化学文摘社编号:

2440-62-2

分子量:

385.37

NACRES:

NA.32

PubChem Substance ID:

24899530

UNSPSC Code:

12352204

EC Number:

219-472-6

MDL number:

MFCD00047721

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属性

产品名称

N-琥珀酰- L -苯丙氨酸- p -硝基苯胺, protease substrate

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

dioxane: 25 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

OC(=O)CCC(=O)N[C@@H](Cc1ccccc1)C(=O)Nc2ccc(cc2)N(=O)=O

InChI

1S/C19H19N3O6/c23-17(10-11-18(24)25)21-16(12-13-4-2-1-3-5-13)19(26)20-14-6-8-15(9-7-14)22(27)28/h1-9,16H,10-12H2,(H,20,26)(H,21,23)(H,24,25)/t16-/m0/s1

InChI key

KNBLWBFJHZHYFG-INIZCTEOSA-N

说明

General description

据报道，这是一种糜蛋白酶的底物。

Application

N -琥珀酰- L -苯丙氨酸- p -硝基苯胺已被用作糜蛋白酶水解活性的底物和摩尔吸光实验。也用于体外监测 α-糜蛋白酶抑制试验。

Biochem/physiol Actions

糜蛋白酶水解活性的底物 N -琥珀酰- L -苯丙氨酸- p -硝基苯胺形成黄色生色团 p -硝基苯胺，可在 410 nm 处分光光度法测定。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Effect of crowding by dextrans on the hydrolysis of N-Succinyl-L-phenyl-Ala-p-nitroanilide catalyzed by α-chymotrypsin.

Isabel Pastor et al.

The journal of physical chemistry. B, 115(5), 1115-1121 (2010-12-31)

Traditionally, studies on the diffusion-controlled reaction of biological macromolecules have been carried out in dilute solutions (in vitro). However, in an intracellular environment (in vivo), there is a high concentration of macromolecules, which results in nonspecific interactions (macromolecular crowding). This

[Solid-phase enzymatic reactions. V. Comparison of catalytic properties of subtilisin and alpha-chymotrypsin in the absence of a solvent].

E Iu Maksareva et al.

Bioorganicheskaia khimiia, 21(1), 24-27 (1995-01-01)

Subtilisin Carlsberg (E.C. 3.4.21.14) catalyzes the hydrolysis of N-succinyl-L-phenylalanine p-nitroanilide in solid-state solvent-free hydrated protein-substrate mixtures. This process needs a certain critical degree of hydration of the protein molecule which is attained at the relative water vapour pressure (p/ps) above

Cyclopeptide alkaloids of Ziziphus oxyphylla Edgw as novel inhibitors of alpha-glucosidase enzyme and protein glycation

Choudhary MI, et al.

Phytochemistry Letters, 4(4), 404-406 (2011)

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全球贸易项目编号

货号	GTIN
S2628-1G	04061836920067
S2628-250MG	04061834742517
S2628-500MG	04061834742524
S2628-5G	04061832925400