Serum Replacement 3 (50×)

Sigma′s Serum Replacement 3 is a fortified, multipurpose serum replacement for the long-term culturing of anchorage dependant and suspension cultures. This serum replacement is effective in research situations such as: studying the effects of growth factors or in the manufacturing of cell derived products.

Serum Replacement 3 (50×) has been used as a supplement in modified phosphate-buffered saline (DPBS) and as a replacement for the growth medium for in vitro experiments for Tendon cells.

Stable for 30 days when diluted in cell culture medium.

Defined serum replacement designed primarily for the long-term growth of human cells or other mammalian cells. Recommended for use in the production of cell-secreted proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, human recombinant insulin). Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells.

This product is tested and found non-reactive for Hepatitis B Surface Antigen (HBsAg) and non-reactive for Human Immunodeficiency Virus (HIV) antibody by ELISA. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly.