Application
样品缓冲液，Laemmli 2x 浓缩液已在SDS-PAGE中被用作一种蛋白样品变性和上样的样品缓冲液。
Biochem/physiol Actions
Laemmli样品缓冲液是经特殊配方用于Lammli SDS-PAGE系统中蛋白样品的制备。 蛋白样品与2X 样品缓冲液（1:1）混合并在沸水中加热2-5分钟。2-巯基乙醇可还原分子内和分子间的二硫键。 SDS去污剂可使蛋白质和亚基变性，并给每个蛋白质和亚基带来整体的负电荷，以便对它们每个基于大小进行分离。 溴酚蓝可作为一种跑在蛋白前端的染料，也可在上样过程中更加容易的看到样品。 甘油可提高样品的密度以便样本能够铺在样品孔中。
Other Notes
含有4% SDS、20% glycerol、10% 2-巯基乙醇、0.004% 溴酚蓝和0.125 M Tris HCl、pH约6.8的溶液。
Preparation Note
推荐使用方法：将1倍体积的凝胶上样溶液加入至1倍体积的样品中并充分混匀。
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
存储类别
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Genetic removal of matrix metalloproteinase 9 rescues the symptoms of fragile X syndrome in a mouse model.
Sidhu H, et al.
The Journal of Neuroscience, 34, 9867-9879 (2014)
Amanda M Gonzales et al.
Nutrition & metabolism, 5, 17-17 (2008-06-14)
Adipocytes express inflammatory mediators that contribute to the low-level, chronic inflammation found in obese subjects and have been linked to the onset of cardiovascular disorders and insulin resistance associated with type 2 diabetes mellitus. A reduction in inflammatory gene expression
GADD45A and EPB41 as tumor suppressor genes in meningioma pathogenesis.
Piaskowski S, et al.
Cancer Genetics and Cytogenetics, 162, 63-67 (2005)
Dana E Nuti et al.
mBio, 2(4), doi:10-doi:10 (2011-08-19)
Detection of microbial antigens in clinical samples can lead to rapid diagnosis of an infection and administration of appropriate therapeutics. A major barrier in diagnostics development is determining which of the potentially hundreds or thousands of antigens produced by a
Maria Marín et al.
Genes, 10(1) (2019-01-20)
XPF endonuclease is one of the most important DNA repair proteins. Encoded by XPF/ERCC4, XPF provides the enzymatic activity of XPF-ERCC1 heterodimer, an endonuclease that incises at the 5' side of various DNA lesions. XPF is essential for nucleotide excision
实验方案
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
相关内容
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持