S4195
β-分泌酶 人
recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), extracellular domain, ≥10,000 units/mg protein, ≥90% (SDS-PAGE), buffered aqueous solution
别名:
BACE1, Memapsin-2, 天冬氨酸-2
产品名称
β-分泌酶 人, recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), ≥10,000 units/mg protein
recombinant
expressed in HEK 293 cells (C-terminal FLAG tagged)
description
extracellular domain
assay
≥90% (SDS-PAGE)
form
buffered aqueous solution
specific activity
≥10,000 units/mg protein
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
human ... BACE1(23621)
Application
人 β-分泌酶或BACE1已被用于研究淀粉样蛋白前体蛋白与β-分泌酶之间的结合反应。 它也已被用于BACE1分析。
Biochem/physiol Actions
BACE1（β-分泌酶或β-位点APP裂解酶）是一种跨膜蛋白酶，负责淀粉样前体蛋白（APP）的β位点裂解，从而产生淀粉样 β 淀粉样蛋白肽（Aβ）。大脑中Aβ的积累是阿尔茨海默氏症进展的主要原因，因此BACE1是发现抑制剂药物的靶标。
BACE1（β位APP裂解酶1）是一种跨膜天冬氨酰蛋白酶，负责裂解淀粉样前体蛋白 (APP)。BACE1介导的淀粉样前体蛋白 (APP)的裂解是致病性淀粉样β肽生成过程中的第一步，因此使其成为阿尔茨海默氏病 (AD)的主要药物靶标。在患有轻度认知障碍（MCI）和AD的患者的脑脊液中发现BACE1的酶活性水平升高，从而表明BACE1酶活性在MCI向AD转化中的重要性。
跨膜蛋白酶负责淀粉样蛋白前体蛋白(APP)的β位点裂解，从而产生淀粉样蛋白β肽。
Physical form
溶于 20 mM Hepes，125 mM NaCl (pH 7.4中。
Other Notes
在37℃温度，pH4.5下，一个单位1分钟将水解1.0甲酚7-甲氧基香豆素-4-乙酰基-[Asn670, Leu671]--淀粉样β/ A4前体蛋白770片段667-676-（2,4-二硝基苯基）-Lys-Arg -Arg酰胺底物。
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Platelet-Derived Secreted Amyloid-Precursor Protein-? as a Marker for Diagnosing Alzheimer?s Disease
Current neurovascular research (2013)
Platelet-Derived Secreted Amyloid-Precursor Protein-? as a Marker for Diagnosing Alzheimer?s Disease
Josef M and Christian H
Current neurovascular research (2013)
Carlo Cervellati et al.
GeroScience, 42(1), 159-167 (2019-11-21)
Beta-secretase (BACE1) is a key enzyme in the formation of amyloid-β; its activity/concentration is increased in brain and cerebrospinal fluid of patients with late-onset Alzheimer's disease (LOAD). Since BACE1 was found also in blood, we evaluated its potential as peripheral
Roland Nicsanu et al.
Journal of Alzheimer's disease : JAD, 87(1), 433-441 (2022-03-12)
Beta-site APP cleaving enzyme 1 (BACE1) is the rate-limiting enzyme in amyloid-β (Aβ) plaques formation. BACE1 activity is increased in brains of patients with Alzheimer's disease (AD) and mild cognitive impairment (MCI) and plasma levels of BACE1 appears to reflect
Function, therapeutic potential and cell biology of BACE proteases: current status and future prospects.
Vassar R
Journal of Neurochemistry, 130(1), 4-28 (2014)
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