产品名称
淀粉样蛋白前体β，分泌型 人, recombinant, expressed in E. coli (N-terminal histidine tagged), solution
recombinant
expressed in E. coli (N-terminal histidine tagged)
form
solution
mol wt
~100 kDa by SDS-PAGE
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... APP(351)
Application
本品人淀粉样前体蛋白β已用作肽标准品。
Biochem/physiol Actions
分泌型β淀粉样蛋白前体(sAPPβ)是β淀粉样蛋白前体(APP)水解得到的裂解产物。APP被β-分泌酶切成2个片段，sAPPβ和β-淀粉样肽(Aβ)。Aβ是阿尔茨海默病(AD)的主要标志之一——淀粉样老年斑的成分，sAPPβ被认为可调节神经元功能和细胞存活。瑞典家系AD中存在的APP突变使APP更易被β-分泌酶切割，形成更多的Aβ和sAPPβ。相反，蛋白激酶C(PKC)激活可降低sAPPβ浓度。反面高尔基体网状结构(TGN)是β-分泌酶活性发挥的主要位点。
General description
淀粉样蛋白-β(Aβ)肽是阿尔茨海默病大脑的老年斑特征的主要成分。它是一种I型跨膜蛋白。编码淀粉样蛋白-β的基因定位于人21号染色体上。
Physical form
0.2 μm过滤溶液，含20 mM Tris(pH 7.4)、0.5 M NaCl、1 mM EDTA、5 mM βME、2 μg/ml抑肽酶
Preparation Note
sAPPβ在大肠杆菌中表达为可溶性蛋白，并在非变性条件下纯化。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering.
Zhang-Haagen B
PLoS ONE (2016)
The Processing of Amyloid Precursor Protein in the
Central Nervous System of Humans and Rhesus
Macaques
Central Nervous System of Humans and Rhesus
Macaques
Justyna Anna Dobrowolska
The West Virginia Medical Journal (2013)
D J Selkoe
Nature, 399(6738 Suppl), A23-A31 (1999-07-07)
Studies of the molecular basis of Alzheimer's disease exemplify the increasingly blurred distinction between basic and applied biomedical research. The four genes so far implicated in familial Alzheimer's disease have each been shown to elevate brain levels of the self-aggregating
Regulation of global gene expression and cell proliferation by APP.
Wu Y
Scientific Reports (2016)
In vivo BACE1 inhibition leads to brain A? lowering and increased a-secretase processing of APP without effect on Neuregulin-1
Sethu Sankaranarayanan
Journal of Pharmacology and Experimental Therapeutics (2018)
相关内容
Datasheet
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