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安全信息

S4320

Sigma-Aldrich

SYBR® Green Extract-N-Amp™ PCR ReadyMix™

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

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NACRES:
NA.31

质量水平

200

用途

sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
 sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
 sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications

特点

dNTPs included
hotstart

detection method

SYBR® Green

运输

wet ice

储存温度

−20°C

相关类别

一般描述

The SYBR® Green Extract-N-Amp™ PCR ReadyMix is the specially formulated PCR enzyme blend contained in the SYBR Green Extract-N-Amp Plant PCR kit and SYBR Green Extract-N-Amp Tissue PCR Kit. It is intended to supplement these kits for researchers who perform more than one amplification per extracted sample.

应用

SYBR® Green Extract-N-Amp™ PCR ReadyMix™ or SyBr Green 2× Master Mix kit has been used in a real-time quantitative polymerase chain reaction (qRT–PCR).

包装

1.2 mL is sufficient for 100 amplifications as described in kit protocols (20 μl total reaction volume)

12 mL is sufficient for 1000 amplifications as described in kit protocols (20 μl total reaction volume)

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,994,056 and 6,171,785.. 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的，仅此等数量的产品用于购买者内部研究用途的的免责许可。我们未明确表示、暗示或以禁止反言的形式授予您其他专利声明下的任何权利（如美国专利号6,814,934号下的设备和系统专利声明）、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

相关产品

产品编号
说明
价格

XNAP

REDExtract-N-Amp™植物PCR试剂盒, sufficient for 100 extractions, sufficient for 100 amplifications

XNAPE

REDExtract-N-Amp™植物PCR试剂盒, sufficient for 100 extractions, sufficient for 500 amplifications

XNAP2

Extract-N-Amp™植物PCR试剂盒, sufficient for 100 extractions, sufficient for 100 amplifications

XNAR

Extract-N-Amp™植物PCR试剂盒, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPR

REDExtract-N-Amp™植物PCR试剂盒, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPS

REDExtract-N-Amp™植物PCR试剂盒, sufficient for 10 extractions, sufficient for 10 amplifications

XNAT2

Extract-N-Amp ™ 组织 PCR 试剂盒, sufficient for 100 extractions, sufficient for 100 amplifications

XNAT2R

Extract-N-Amp ™ 组织 PCR 试剂盒, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAT

REDExtract-N-Amp™ 组织PCR试剂盒, sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

R4775

REDExtract-N-Amp™ PCR ReadyMix™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

提取-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E8155

Extract-N-Amp™ PCR Diluent, colorless, storage temp.:2-8°C

E7526

提取溶液, Molecular Biology

D5688

Dilution Solution, Extract-N-Amp Plant Dilution Solution

XNAPG

SYBR® Green Extract-N-Amp™植物PCR试剂盒, sufficient for 100 preparations

XNATG

SYBR® Green Extract-N-Amp™组织PCR试剂盒, sufficient for 100 extractions, sufficient for 100 amplifications

T3073

组织制备溶液, 30 mL sufficient for 1,000 extractions

E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

法规信息

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Sudhakar Srivastava et al.
Journal of experimental botany, 64(1), 303-315 (2012-11-20)
MicroRNAs (miRNAs) constitute a novel mechanism of gene regulation affecting plant development, growth, and stress response. To study the role of miRNAs in arsenic (As) stress, microarray profiling of miRNAs was performed in Brassica juncea using a custom Phalanx Plant
Vikas Yadav Patade et al.
Protoplasma, 248(3), 613-621 (2010-09-21)
Identification of genes whose expression enables plants to adapt to any kind of stresses is integral to developing stress tolerance in crop plants. In this study, PCR-based cDNA suppression subtractive hybridization technique was used to construct sugarcane salt (NaCl) stress
Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
Ririe KM, Rasmussen RP, Wittwer CT
Analytical Biochemistry, Feb, 154-160 (1997)
Karuna Yadav et al.
PloS one, 12(11), e0188933-e0188933 (2017-12-01)
Iron is an indispensable element for plant growth and defense and hence it is essential to improve the plant's ability to accumulate iron. Besides, it is also an important aspect for human health. In view of this, we attempted to
Archana N Rai et al.
3 Biotech, 10(3), 114-114 (2020-03-03)
Heat stress is one of the major limitations to crop productivity. In the present study, an efficient method of screening was adopted for identification of heat tolerant Indian Mustard genotypes by applying 4-day cycle of heat stress to seedlings. Thirty-four genotypes

商品

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

实验方案

SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol

The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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