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Merck
CN

S4320

Sigma-Aldrich

SYBR® Green Extract-N-Amp PCR ReadyMix

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

别名:

SYBR Green PCR Mix

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About This Item

UNSPSC代码:
41106303
NACRES:
NA.31

质量水平

用途

sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications

特点

dNTPs included
hotstart

检测方法

SYBR® Green

运输

wet ice

储存温度

−20°C

一般描述

The SYBR® Green Extract-N-Amp PCR ReadyMix is the specially formulated PCR enzyme blend contained in the SYBR Green Extract-N-Amp Plant PCR kit and SYBR Green Extract-N-Amp Tissue PCR Kit. It is intended to supplement these kits for researchers who perform more than one amplification per extracted sample.

应用

SYBR® Green Extract-N-Amp PCR ReadyMix or SyBr Green 2× Master Mix kit has been used in a real-time quantitative polymerase chain reaction (qRT–PCR).

包装

1.2 mL is sufficient for 100 amplifications as described in kit protocols (20 μl total reaction volume)

12 mL is sufficient for 1000 amplifications as described in kit protocols (20 μl total reaction volume)

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,994,056 and 6,171,785.. 购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的,仅此等数量的产品用于购买者内部研究用途的的免责许可。我们未明确表示、暗示或以禁止反言的形式授予您其他专利声明下的任何权利(如美国专利号6,814,934号下的设备和系统专利声明)、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

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Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
Ririe KM, Rasmussen RP, Wittwer CT
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Identification of genes whose expression enables plants to adapt to any kind of stresses is integral to developing stress tolerance in crop plants. In this study, PCR-based cDNA suppression subtractive hybridization technique was used to construct sugarcane salt (NaCl) stress
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MicroRNAs (miRNAs) constitute a novel mechanism of gene regulation affecting plant development, growth, and stress response. To study the role of miRNAs in arsenic (As) stress, microarray profiling of miRNAs was performed in Brassica juncea using a custom Phalanx Plant
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Iron is an indispensable element for plant growth and defense and hence it is essential to improve the plant's ability to accumulate iron. Besides, it is also an important aspect for human health. In view of this, we attempted to
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Heat stress is one of the major limitations to crop productivity. In the present study, an efficient method of screening was adopted for identification of heat tolerant Indian Mustard genotypes by applying 4-day cycle of heat stress to seedlings. Thirty-four genotypes

商品

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

实验方案

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

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