S5193
SYBR™ Green JumpStart™ Taq ReadyMix™
for quantitative PCR, without MgCl2 in buffer
别名:
Hot start PCR master mix, sybr green PCR master mix, sybr green qPCR
选择规格
关于此项目
Quality Segment
form
liquid
usage
sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 400 reactions
feature
dNTPs included, hotstart
concentration
1.25 units/reaction (50 μL reaction volume)
technique(s)
RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable
color
colorless
input
purified DNA
suitability
suitable for PCR
application(s)
agriculture
compatibility
Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ 5, for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7500 Fast, for use with ABI 7500, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI 7900, for use with ABI StepOne, for use with ABI StepOnePlus, for use with ABI ViiA 7, for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MJ Opticon, for use with Bio-Rad MiniOpticon, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Eppendorf® Mastercycler ep realplex, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000
detection method
SYBR™ Green
shipped in
wet ice
storage temp.
−20°C
General description
Application
- During RNA extraction, reverse transcription and Quantitative PCR
- During analysis of leptin and leptin receptor expression. (PCR and melting curve analyses were performed)
- For tissue RNA Isolation. (PCR and melting curve analyses were performed)
- in real-time PCR (RT-PCR) of reverse-transcribed cDNA template
- For qPCR amplifications using SYBR
- For multiplex PCR
Biochem/physiol Actions
SYBR Green I是一种常用的荧光DNA结合染料，可结合所有双链DNA，并通过测量整个循环中荧光的增加来监测检测。SYBR Green I最大激活和发射峰值分别在494nm和521nm。 ROX参考染料仪器设定符合定量PCR参考染料测量需要。 在加入一种热启动介导tag聚合酶，JumpStart Taq后，Sigma′s基于SYBR QPCR检测的特异性被增强。
Features and Benefits
- 通过在ReadyMix中进行SYBR Green检测提供抗体灭活热启动PCR的优势，是高通量应用的理想之选；只需引物和模板即可
- JumpStart™ Taq DNA聚合酶可防止非特异性产物扩增，进而提高效率和靶标得率。
- SYBR™ Green JumpStart™ Taq ReadyMix适用于基于SYBR的qPCR，采用MgCl2配制或采用独立小瓶包装，便于优化。ReadyMixes与管式和板式仪器兼容
- 该预混液可确保反应间一致性
- 可最大限度减少污染
- 减少了引物二聚体
- 与手动或蜡质热启动®方法相比，制备时间更短
- 允许室温构建反应并随附荧光染料；qPCR应用的理想之选
Packaging
20RXN包装为1 X 500μL
100RXN包装为1 X 2.5 mL
400RXN包装为1 X 10 mL
500RXN包装为1 X 12.5 mL
Other Notes
Legal Information
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signalword
Warning
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3