生物来源
bovine fetus
质量水平
表单
frozen liquid
浓度
≥14 mg protein/mL Biuret
技术
cell culture | mammalian: suitable
cell culture | stem cell: suitable
杂质
<1 ng/mg protein endotoxin
<5 % of normal lipoprotein
运输
dry ice
储存温度
−20°C
一般描述
研究表明，使用脂蛋白缺乏血清条件下培养的人成纤维细胞中甾醇的产生减少。
应用
来自胎牛的脂蛋白缺乏血清已被用作培养基中的补充剂，用于培养中国仓鼠卵巢（CHO）衍生细胞、胸腺叶和人外周血单核细胞（PBMC）。
制备说明
无菌过滤， 0.2 μm
用 Dulbecco′s 磷酸盐缓冲盐水（pH 7.4-7.5）进行透析
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
监管及禁止进口产品
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)
P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Ying Wang et al.
Cell death discovery, 7(1), 201-201 (2021-08-06)
Coenzyme Q (CoQ) is a lipid-like mobile electron transporter of the mitochondrial respiratory chain. Patients with partial loss-of-function mutations in the CoQ biosynthesis pathway suffer from partial primary CoQ deficiency (MIM 607426). This leads to mitochondrial dysfunction, which presents like
实验方案
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
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