产品名称
超氧化物歧化酶 来源于牛红细胞, BioReagent, ≥3,000 units/mg protein, suitable for cell culture, lyophilized powder
biological source
bovine erythrocytes
product line
BioReagent
form
lyophilized powder
specific activity
≥3,000 units/mg protein
mol wt
32.5 kDa
packaging
pkg of 15000 units
technique(s)
cell culture | mammalian: suitable
pH
7.6-10.5
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Analysis Note
关于试验方法请参阅 McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969)。
Application
牛红细胞来源超氧化物歧化酶(SOD)已用于:
- 通过人脑微血管内皮细胞中的电子顺磁共振自旋,测定超氧化物自由基
- 通过细胞色素C测定,测定外周血单核细胞中的超氧化物产量
- 用作傅里叶变换红外光谱(FTIR)和基质辅助激光解吸/电离(MALDI)分析法鉴定鸡蛋SOD的标准品
Biochem/physiol Actions
牛红细胞来源的超氧化物歧化酶可催化超氧化物自由基歧化为过氧化氢和分子氧。作为一种抗氧化剂,它在保护细胞免受氧自由基毒性作用方面起着至关重要的作用。与一氧化氮(NO)竞争超氧化物阴离子(可与NO反应生成过亚硝酸盐),因此SOD能促进NO的活性。在培养的大鼠卵泡、神经细胞系和转基因小鼠中,SOD也表现出抑制细胞凋亡的作用。
General description
牛红细胞来源超氧化物歧化酶是一种金属蛋白,可超氧化物阴离子自由基歧化。这是一种31.5 kDa的铜结合蛋白,呈现保守的结构域和折叠。它是一种同源二聚体,每个亚基具有一个铜和锌离子,且具有反平行“希腊回纹”β桶状折叠。
Other Notes
在pH 7.8,25℃条件下,3.0mL反应体积中,一个单位可在黄嘌呤氧化酶的偶联系统中抑制细胞色素c的减少达到50%。黄嘌呤氧化酶浓度应产生初始ΔA550 =0.025±0.005/分钟。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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实验方案
Enzymatic Assay of Superoxide Dismutase
Separation of Superoxide dismutase
在测定超氧化物歧化酶活性时,使用了一种在550 nm处的连续分光光度法进行测定。一个酶单位可将酶偶联系统的细胞色素c还原率抑制50%。
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