S5816
Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse
>97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture
别名:
SDF-1α/PBSF
产品名称
Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse, SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
mouse
recombinant
expressed in E. coli
assay
>97% (SDS-PAGE)
form
powder
potency
0.15-0.6 ng/mL ED50
quality
endotoxin tested
mol wt
predicted mol wt ~8 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μgtested (LAL test)
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
mouse ... Cxcl12(20315)
Analysis Note
The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.
Application
Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.
Biochem/physiol Actions
SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..
Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.
General description
Stromal derived factor-1 (SDF-1) is localized to adipocytes.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.
Preparation Note
Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
SDF-1α-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia
Liu ZJ, et al.
Scientific Reports, 6: 34416 (2016)
SDF-1 Is an Autocrine Insulin-Desensitizing Factor in Adipocytes.
Shin J, et al.
Diabetes, 67(6), 1068-1078 (2018)
The Cytokine CXCL12 Promotes Basket Interneuron Inhibitory Synapses in the Medial Prefrontal Cortex.
Wu PR, et al.
Cerebral Cortex, 27(9), 4303-4313 (2017)
CXCR4 receptor positive spheroid forming cells are responsible for tumor invasion in vitro
Krohn A, et al.
Cancer Letters, 280, 65-71 (2009)
Mobilization of mesenchymal stem cells by stromal cell-derived factor-1 released from chitosan/tripolyphosphate/fucoidan nanoparticles
Huang YC, et al.
Acta Biomaterialia, 8, 1048-1056 (2012)
相关内容
Datasheet
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