S5816
Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse
>97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture
别名:
SDF-1α/PBSF
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关于此项目
Biological source:
mouse
Recombinant:
expressed in E. coli
Assay:
>97% (SDS-PAGE)
Form:
powder
Mol wt:
predicted mol wt ~8 kDa
Impurities:
<1 EU/μgtested (LAL test)
产品名称
Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse, SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
mouse
recombinant
expressed in E. coli
assay
>97% (SDS-PAGE)
form
powder
potency
0.15-0.6 ng/mL ED50
quality
endotoxin tested
mol wt
predicted mol wt ~8 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μgtested (LAL test)
UniProt accession no.
storage temp.
−20°C
Quality Level
General description
Stromal derived factor-1 (SDF-1) is localized to adipocytes.
Application
Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.
Biochem/physiol Actions
SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..
Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.
Preparation Note
Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.
Analysis Note
The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
SDF-1 Is an Autocrine Insulin-Desensitizing Factor in Adipocytes.
Shin J, et al.
Diabetes, 67(6), 1068-1078 (2018)
SDF-1α-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia
Liu ZJ, et al.
Scientific Reports, 6: 34416 (2016)
CXCR4 receptor positive spheroid forming cells are responsible for tumor invasion in vitro
Krohn A, et al.
Cancer Letters, 280, 65-71 (2009)
Mobilization of mesenchymal stem cells by stromal cell-derived factor-1 released from chitosan/tripolyphosphate/fucoidan nanoparticles
Huang YC, et al.
Acta Biomaterialia, 8, 1048-1056 (2012)
The Cytokine CXCL12 Promotes Basket Interneuron Inhibitory Synapses in the Medial Prefrontal Cortex.
Wu PR, et al.
Cerebral Cortex, 27(9), 4303-4313 (2017)
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