产品名称
葡聚糖凝胶 ® G-50, BioReagent, DNA grade, Molecular Biology, fine
SMILES string
O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
grade
Molecular Biology
product line
BioReagent
form
beads
technique(s)
DNA purification: suitable
matrix
cross-linked dextran
swelling
1 g swells to 9-11 mL
bead diameter
20-80 μm
pore size
exclusion limit (20 bp dsDNA (rA)20)
pH
2-10
foreign activity
DNase, RNase, none detected
storage temp.
room temp
Quality Level
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Application
塞法戴克斯 ® G-50 适用于离心柱纯化 DNA（来自污染的小分子），用于杂交。用于 DNA 测序前纯化 PCR 产物，以提高测序质量。
Features and Benefits
- 极佳的回收率
- 最小样品稀释度
- 快速且易于使用
General description
塞法戴克斯 ® G-50,Fine 是一种常用的凝胶过滤介质，用于 >30,000 MW 生物分子的脱盐和缓冲液交换。塞法戴克斯®通过葡聚糖与环氧氯丙烷交联制备。塞法戴克斯的交联程度不同，因此其溶胀程度和分子分馏范围也不同。“Fine”表示具有极好的效率和较短的扩散距离的小粒径。
S5897-100G 的更新产品编号为 GE17-0573-02
S5897-100G 的更新产品编号为 GE17-0573-02
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Aron Fazekas et al.
BioTechniques, 48(4), 277-285 (2010-06-24)
Stutter products are a common artifact in the PCR amplification of frequently used genetic markers that contain mononucleotide simple sequence repeats. Despite the importance of accurate determination of nucleotide sequence and allele size, there has been little progress toward decreasing
Torben Riehl et al.
PloS one, 7(11), e49354-e49354 (2012-11-13)
The Amundsen Sea, Antarctica, is amongst the most rapidly changing environments of the world. Its benthic inhabitants are barely known and the BIOPEARL 2 project was one of the first to biologically explore this region. Collected during this expedition, Macrostylis
Katharina Besemer et al.
Applied and environmental microbiology, 75(22), 7189-7195 (2009-09-22)
Streams are highly heterogeneous ecosystems, in terms of both geomorphology and hydrodynamics. While flow is recognized to shape the physical architecture of benthic biofilms, we do not yet understand what drives community assembly and biodiversity of benthic biofilms in the
Ryo Takeuchi et al.
Nucleic acids research, 37(3), 877-890 (2008-12-24)
The LAGLIDADG homing endonuclease (LHE) I-AniI has adopted an extremely efficient secondary RNA splicing activity that is beneficial to its host, balanced against inefficient DNA cleavage. A selection experiment identified point mutations in the enzyme that act synergistically to improve
Mark Del Campo et al.
Journal of molecular biology, 389(4), 674-693 (2009-04-28)
The DEAD-box proteins CYT-19 in Neurospora crassa and Mss116p in Saccharomyces cerevisiae are broadly acting RNA chaperones that function in mitochondria to stimulate group I and group II intron splicing and to activate mRNA translation. Previous studies showed that the
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