产品名称
葡聚糖凝胶 ® G-100, DNA grade, BioReagent, Molecular Biology, medium
grade
Molecular Biology
product line
BioReagent
form
beads
technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 9-11 mL
bead diameter
40-120 μm
pore size
exclusion limit (25 bp dsDNA; (rA)27)
pH
2-10
application(s)
life science and biopharma
compatibility
Cytiva
foreign activity
DNase, RNase, none detected
storage temp.
room temp
Quality Level
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Application
Sephadex ® G-100 适用于离心柱纯化 DNA（来自污染的小分子），用于杂交。采用凝胶过滤法分离纯化香蕉中多酚氧化酶。
Features and Benefits
- 极佳的回收率
- 最小样品稀释度
- 快速且易于使用
General description
Sephadex® G-100葡聚糖凝胶（中级）是经常被文献引用的凝胶过滤基质，主要用于超大分子的脱盐和缓冲液置换。 Sephadex®葡聚糖凝胶由葡聚糖(dextran)与环氧氯丙烷交联而成。Sephadex产品有各种不同的交联度，因此其溶胀程度和分子分离范围也不同。 “中级”代表较大颗粒，主要用于要求高流速和低操作压力的大规模组群分离。
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
Galeazzi MAM et al
Journal of Food Science, 46, 150-155 (1981)
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
S Kaushik et al.
Methods in molecular biology (Clifton, N.J.), 445, 227-244 (2008-04-22)
Chaperone-mediated autophagy (CMA) is the only type of autophagy in mammalian cells able to selectively degrade cytosolic proteins in lysosomes. CMA is maximally activated in response to stressors such as prolonged starvation, exposure to toxic compounds, or oxidative stress. We
Zhipeng Li et al.
International immunopharmacology, 14(2), 145-149 (2012-07-10)
The study is to investigate the effect of compound IMMLG5521, a coumarin derivative, on lung inflammation induced by Sephadex in rats. Sephadex led to massive granulomas, infiltration of neutrophils and eosinophils, the increase of TNF-α level in BALF and lung
Veronica M Chiu et al.
Analytical biochemistry, 418(1), 58-65 (2011-08-02)
An unstirred layer (USL) exists at the interface of solids with solutions. Thus, the particles in brain tissue preparations possess a USL as well as at the surface of a rotating disk electrode (RDE) used to measure chemical fluxes. Time
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