一般描述
Sephadex® G-100葡聚糖凝胶（中级）是经常被文献引用的凝胶过滤基质，主要用于超大分子的脱盐和缓冲液置换。 Sephadex®葡聚糖凝胶由葡聚糖(dextran)与环氧氯丙烷交联而成。Sephadex产品有各种不同的交联度，因此其溶胀程度和分子分离范围也不同。 “中级”代表较大颗粒，主要用于要求高流速和低操作压力的大规模组群分离。
应用
Sephadex ® G-100 适用于离心柱纯化 DNA（来自污染的小分子），用于杂交。采用凝胶过滤法分离纯化香蕉中多酚氧化酶。
特点和优势
- 极佳的回收率
- 最小样品稀释度
- 快速且易于使用
法律信息
Sephadex is a registered trademark of Cytiva
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
Galeazzi MAM et al
Journal of Food Science, 46, 150-155 (1981)
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
S Kaushik et al.
Methods in molecular biology (Clifton, N.J.), 445, 227-244 (2008-04-22)
Chaperone-mediated autophagy (CMA) is the only type of autophagy in mammalian cells able to selectively degrade cytosolic proteins in lysosomes. CMA is maximally activated in response to stressors such as prolonged starvation, exposure to toxic compounds, or oxidative stress. We
Sanjiv K Mishra et al.
Protein expression and purification, 80(2), 234-238 (2011-07-13)
C-phycoerythrin was isolated and purified from marine Pseudanabaena sp. using two step chromatographic methods. Phycobiliproteins in the marine Pseudanabaena was extracted in 100 mM phosphate buffer (pH 7.2) and precipitated by salting out. The precipitated C-phycoerythrin was purified by gel
Fernanda O Lemos et al.
European journal of dermatology : EJD, 21(5), 722-730 (2011-07-09)
Previous studies demonstrated that proteinases from latex of C. candamarcensis act as mitogens on fibroblast and epithelial cells and a subsequent report showed their protective, angiogenic and wound healing effects on gastric ulcers. In this study, we present evidence of
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