General description
Spectinomycin is an aminocyclitol antibiotic naturally produced by Streptomyces spectabilis. Spectinomycin shows in vitro inhibitory effect (31.2μg/mL) against gram-negative and gram-positive bacteria like Escherichia coli, Klebsiella, Enterobacter, and Staphylococcus epidermidis. It acts as a protein synthesis inhibitor by binding to the bacterium′s 30S ribosomal subunit. Specifically, it interacts with 16S rRNA during the elongation process and blocks elongation factor G (EF-G), which is responsible for the translocation catalysis of peptidyl-tRNA.
Spectinomycin is used as a selective resistance marker (Spc) in Leptospira spp. The Spc marker can be used for transformation screening in leptospires from both plasmids or chromosomal integration. Spectinomycin resistance occurs through mutation of rpsE gene which encodes the protein S5, or via the aadA bacterial gene which encodes the enzyme Aminoglycoside-3"-adenyltransferase. Spectinomycin can be also used in amplification of low copy number plasmid carrying replicons (100μg/mL).
In the clinic, Spectinomycin is used as a treatment against Neisseria gonorrhoeae for both gonorrheal urethritis and cervictis.
Spectinomycin is also a plastid protein synthesis inhibitor. It is used as a specific marker (0.2-5 mg/mL) for cell layer to monitor leaf development, and is also used to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media (0.5mg/mL).Spectinomycin was also studied for cattle respiratory tract infections by Pasteurella multocida and Mannheimia haemolytica.
Technical information
1. Spectinomycin ready made solution is formulated in order to prevent freezing at -20°C, and to avoid solution precipitation under freeze-thaw cycles.
2.The suggested concentration of Spectinomycin (based on literature) in cell culture assays is 0.2-100mγ/mL. Therefore, it is recommended to dilute the ready made solution 1:1000-500,000.
Spectinomycin is used as a selective resistance marker (Spc) in Leptospira spp. The Spc marker can be used for transformation screening in leptospires from both plasmids or chromosomal integration. Spectinomycin resistance occurs through mutation of rpsE gene which encodes the protein S5, or via the aadA bacterial gene which encodes the enzyme Aminoglycoside-3"-adenyltransferase. Spectinomycin can be also used in amplification of low copy number plasmid carrying replicons (100μg/mL).
In the clinic, Spectinomycin is used as a treatment against Neisseria gonorrhoeae for both gonorrheal urethritis and cervictis.
Spectinomycin is also a plastid protein synthesis inhibitor. It is used as a specific marker (0.2-5 mg/mL) for cell layer to monitor leaf development, and is also used to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media (0.5mg/mL).Spectinomycin was also studied for cattle respiratory tract infections by Pasteurella multocida and Mannheimia haemolytica.
Technical information
1. Spectinomycin ready made solution is formulated in order to prevent freezing at -20°C, and to avoid solution precipitation under freeze-thaw cycles.
2.The suggested concentration of Spectinomycin (based on literature) in cell culture assays is 0.2-100mγ/mL. Therefore, it is recommended to dilute the ready made solution 1:1000-500,000.
Physical form
Liquid - in 1:1 DMSO:water
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Alain Brans et al.
Applied and environmental microbiology, 70(12), 7241-7250 (2004-12-03)
The novel method described in this paper combines the use of blaI, which encodes a repressor involved in Bacillus licheniformis BlaP beta-lactamase regulation, an antibiotic resistance gene, and a B. subtilis strain (BS1541) that is conditionally auxotrophic for lysine. We
I I Washington JA et al.
Antimicrobial agents and chemotherapy, 2(6), 427-430 (1972-12-01)
The in vitro inhibitory and bactericidal activities of spectinomycin hydrochloride were tested against a variety of bacteria. The antibiotic was inhibitory at 31.2 mug/ml to most strains of Escherichia coli, Klebsiella, Enterobacter, and Staphylococcus epidermidis. Concentrations of antibiotic exhibiting bactericidal
Localization of the amino-acid exchange in protein S5 from an Escherichia coli mutant resistant to spectinomycin.
M DeWilde et al.
Molecular & general genetics : MGG, 127(3), 273-276 (1973-12-31)