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Merck
CN

S7571

超氧化物歧化酶 来源于牛红细胞

greener alternative

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

别名:

红细胞铜蛋白, 细胞铜蛋白, 血铜蛋白, 超氧化物歧化酶1, 牛, 铜/锌-SOD, SOD, 超氧化物:超氧化物氧化还原酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-943-0
MDL number:
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产品名称

超氧化物歧化酶 来源于牛红细胞, lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

biological source

bovine

form

lyophilized powder

specific activity

≥3,000 units/mg protein

mol wt

32.5 kDa

composition

Protein, ≥95% biuret

storage condition

(Store under nitrogen.
Tightly closed. Dry.)

greener alternative product characteristics

Atom Economy
Design for Energy Efficiency
Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

immunoblotting: suitable
inhibition assay: suitable

color

blue-green

pI 

4.95

solubility

water: 20 mg/mL
aqueous buffer, pH 7.5: soluble

UniProt accession no.

application(s)

diagnostic assay manufacturing

greener alternative category

storage temp.

−20°C

Quality Level

Gene Information

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相关类别

Physical form

冻干粉末,基本上不含盐

Application

红细胞超氧化物歧化酶已用于:

  • 在一项研究评估辐射诱导失活的超氧化物歧化酶在一氧化二氮饱和溶液中的动力学模型
  • 研究超氧阴离子参与肠色氨酸2,3-双加氧酶反应的可能性
  • 调查其对人红细胞溶血率及红细胞血红蛋白-一氧化氮复合物(HbNO)稳定性的影响
  • 联合过氧化氢酶体外研究其对细胞分化的影响
  • 通过化学发光和细胞色素C还原法量化超氧化物水平,研究其对小鼠肺动脉反应性的影响

Biochem/physiol Actions

牛红细胞来源的超氧化物歧化酶可催化超氧化物自由基歧化为过氧化氢和分子氧。作为一种抗氧化剂,它在保护细胞免受氧自由基毒性作用方面起着至关重要的作用。与一氧化氮(NO)竞争超氧化物阴离子(可与NO反应生成过亚硝酸盐),因此SOD能促进NO的活性。在培养的大鼠卵泡、神经细胞系和转基因小鼠中,SOD也表现出抑制细胞凋亡的作用。

General description

研究领域:细胞信号传导

超氧化物歧化酶(SOD)是一种具有氧化还原活性的金属酶,在有氧和厌氧生物体内均有表达。牛超氧化物歧化酶或CuZn SOD是一种同源二聚体,每个亚基含有一个锌离子和一个铜离子。

Other Notes

在 3.0ml 反应体系、25℃、pH 7.8 条件下,在与黄嘌呤氧化酶的偶联系统中,一个单位将抑制 50% 的细胞色素 c 的还原。黄嘌呤氧化酶浓度应每分钟产生 0.025±0.005 的初始 ΔA550

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Tomohiro Furukawa et al.
Toxins, 11(2) (2019-02-15)
Aflatoxin contamination of crops is a worldwide problem, and elucidation of the regulatory mechanism of aflatoxin production, for example relative to the oxidative⁻antioxidative system, is needed. Studies have shown that oxidative stress induced by reactive oxygen species promotes aflatoxin production.
Michael A Hough et al.
Structure (London, England : 1993), 11(8), 937-946 (2003-08-09)
Copper zinc superoxide dismutase (CuZnSOD) forms a crucial component of the cellular response to oxidative stress by catalyzing the dismutation of the superoxide radical to hydrogen peroxide and water. Mutations in human CuZnSOD are associated with the development of familial
Chenting Zhu et al.
Translational research : the journal of laboratory and clinical medicine, 152(6), 273-282 (2008-12-09)
Ischemia reperfusion (I/R) injury of the lung affects the function of the nonischemic lung. Our objective is to determine how apocynin, which is a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, protects the nonischemic control right lung (RL) from injury
Vera G Grivennikova et al.
Redox biology, 17, 192-199 (2018-04-28)
The initial rates of superoxide plus hydrogen peroxide (ROS) generation by intact or permeabilized rat heart mitochondria and coupled inside-out bovine heart submitochondrial particles (SMP) oxidizing NAD-dependent substrates, NADH, and succinate were measured by detecting resorufin formation in the Amplex
C A Chuaqui et al.
Biochimica et biophysica acta, 702(1), 112-116 (1982-03-18)
It has been reported that the inactivation yield of superoxide dismutase from bovine erythrocytes irradiated in N2O-saturated solutions increases exponentially with the initial enzyme concentration and that dose-response curves are non-exponential. The present work analyses these data using a kinetic

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Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

氧化应激,在一部分上,是由多种细胞过程产生的活性氧介导,并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基,如活性氧,会通过细胞引起细胞损伤。

实验方案

Enzymatic Assay of Superoxide Dismutase

在测定超氧化物歧化酶活性时,使用了一种在550 nm处的连续分光光度法进行测定。一个酶单位可将酶偶联系统的细胞色素c还原率抑制50%。

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