S7945
Monoclonal Anti-Siah2 antibody produced in mouse
clone Siah2-369, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Seven in Absentia Homolog
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关于此项目
Conjugate:
unconjugated
Clone:
Siah2-369, monoclonal
Application:
ARR, ELISA (i), ICC, WB
Citations:
8
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Siah2-369, monoclonal
form
buffered aqueous solution
mol wt
antigen ~37 kDa
species reactivity
monkey, hamster, bovine, mouse, human, canine
technique(s)
immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 2-4 μg/mL using total cell extract of HeLa cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SIAH2(6478)
mouse ... Siah2(20439)
General description
Monoclonal Anti-Siah2 (mouse IgG1 isotype) is derived from the hybridoma Siah2-369 produced by the fusion of mouse myeloma cells (NS1 cell) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 2-17 of human Siah2, conjugated to KLH. Seven in absentia homologue (Siah) is encoded by the gene mapped to human chromosome 3q25.1. Three different Siah genes exist in mice, Siah1a, Siah1b, and Siah2, while humans have only two genes, Siah1and Siah2.
Immunogen
synthetic peptide corresponding to amino acids 2-17 of human Siah2, conjugated to KLH.
Application
Monoclonal Anti-Siah2 antibody produced in mouse can be used in enzyme linked immunoassay (ELISA) and immunocytochemistry. It has also been used in immunohistochemistry and immunoblotting.
Biochem/physiol Actions
Seven in absentia homologue (Siah) plays a role in the regulation of key proteins in the immune system such as TNF receptor associated factors (TRAF), vav guanine nucleotide exchange factor 1 (Vav1), and oct-binding factor 1 (OBF-1). Siah2 is important for the regulation of the PHD enzymes (prolylhydroxylases) that are responsible for the prolyhydroxylation of HIFα protein under hypoxia conditions.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
此项目有
Hypoxia regulates Hippo signaling through the SIAH2 ubiquitin E3 ligase
Ma B, et al.
Nature Cell Biology, 17(1), 95-95 (2015)
Generation and analysis of Siah2 mutant mice
Frew IJ, et al.
Molecular and Cellular Biology, 23(24), 9150-9161 (2003)
Wnt5a induces and maintains prostate cancer cells dormancy in bone
Ren D, et al.
The Journal of Experimental Medicine, 216(2), 428-449 (2019)
A genome-wide association study identifies a genetic variant in the SIAH2 locus associated with hormonal receptor-positive breast cancer in Japanese
Elgazzar S, et al.
Journal of Human Genetics, 57(12), 766-766 (2012)
Prolyl hydroxylase domain enzymes: important regulators of cancer metabolism
Yang M, et al.
Hypoxia (Auckland, N.Z.), 2(24), 127-127 (2014)
相关内容
Datasheet
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