S8160
超氧化物歧化酶 来源于牛肝脏
lyophilized powder, ≥1500 units/mg protein
别名:
SOD, 超氧化物:超氧化物氧化还原酶
产品名称
超氧化物歧化酶 来源于牛肝脏, lyophilized powder, ≥1500 units/mg protein
form
lyophilized powder
specific activity
≥1500 units/mg protein
mol wt
32.5 kDa
composition
Protein, ≥70% biuret
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
cow ... SOD1(281495)
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Analysis Note
关于试验方法请参阅 McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969)。
Application
来自牛肝的超氧化物歧化酶已被用于一项研究中，用于确定高胆固醇血症可以增加内皮细胞超氧化物阴离子的生成。 来自牛肝的超氧化物歧化酶也已被用于研究牛红细胞超氧化物歧化酶的重氮偶联、亚基相互作用和电泳变体。它也被用作O2k 荧光法定量测定活性氧（ROS）的分析缓冲液成分。
Biochem/physiol Actions
超氧化物歧化酶催化超氧化物自由基的歧化反应生成过氧化氢和分子氧。它在细胞防御氧自由基的毒性作用方面发挥着重要作用。它 与一氧化氮(NO)竞争超氧化物阴离子（可与NO反应生成过亚硝酸盐），因此SOD能促进NO的活性。在培养的大鼠卵泡、神经细胞系和转基因小鼠中，SOD也表现出抑制细胞凋亡的作用。
General description
研究领域：细胞信号传导 超氧化物歧化酶（SOD）是一种低分子量蛋白，存在于微生物、植物和动物的所有需氧细胞中。它以三个不同家族的形式存在，如锰SOD、铜–锌SOD和胞外SOD。
Other Notes
在 25℃、pH 7.8 的黄嘌呤氧化酶偶联系统中，1 个单位将抑制细胞色素 c 减少 50%（反应体积 3.0 mL）。黄嘌呤氧化酶浓度应每分钟 产生 0.025±0.005 初始 ΔA 550 。
Physical form
含磷酸钾缓冲盐的冻干粉
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Characterisation by EPR spectroscopy of the co-ordination environment of copper in superoxide dismutase from horseradish (Armoracia rusticana Gaertn.)
Palivan, CG and Palivan, H
Proceedings of the Royal Society of Edinburgh. Section B: Biology, 102, 273-277 (1994)
Y Ohara et al.
The Journal of clinical investigation, 91(6), 2546-2551 (1993-06-01)
Indirect evidence suggests accelerated degradation of endothelium-derived nitric oxide (ENDO) by superoxide anion (O2-) in hypercholesterolemic vessels (HV). To directly measure O2- production by normal vessels (NV) and HV, we used an assay for O2- based on the chemiluminescence (CL)
Bovine erythrocyte superoxide dismutase: diazo coupling, subunit interactions, and electrophoretic variants.
D P Malinowski et al.
Biochemistry, 18(1), 237-244 (1979-01-09)
S D Yan et al.
The Journal of biological chemistry, 269(13), 9889-9897 (1994-04-01)
Attack by reactive oxygen intermediates, common to many kinds of cell/tissue injury, has been implicated in the development of diabetic and other vascular diseases. Such oxygen-free radicals can be generated by advanced glycation end products (AGEs), which are nonenzymatically glycated
Lu Miao et al.
Free radical biology & medicine, 47(4), 344-356 (2009-05-30)
Numerous short-lived and highly reactive oxygen species (ROS) such as superoxide (O2(.-)), hydroxyl radical, and hydrogen peroxide are continuously generated in vivo. Depending upon concentration, location, and intracellular conditions, ROS can cause toxicity or act as signaling molecules. The cellular
实验方案
Enzymatic Assay of Superoxide Dismutase
Separation of Superoxide dismutase
在测定超氧化物歧化酶活性时，使用了一种在550 nm处的连续分光光度法进行测定。一个酶单位可将酶偶联系统的细胞色素c还原率抑制50％。
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