S9754
硫酸脂酶 来源于鲍鱼内脏
Type VIII, lyophilized powder, 20-40 units/mg solid
别名:
芳基硫酸酯酶, 苯硫酸酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-772-1
MDL number:
Specific activity:
20-40 units/mg solid
Biological source:
abalone entrails
Quality Level
biological source
abalone entrails
type
Type VIII
form
lyophilized powder
specific activity
20-40 units/mg solid
mol wt
~43 kDa by gel filtration
foreign activity
β-Glucuronidase ≤10.0 Sigma units/mg solid
storage temp.
−20°C
Application
来自鲍鱼内脏的硫酸酯酶已用于研究中，确定人 p-选择蛋白糖蛋白配体-1 通过 PSGL-1氨基末端的硫酸化酪氨酸与皮肤相关趋化因子 CCL27 相互作用。 来自鲍鱼内脏的硫酸酯酶也已用于研究来自仓鼠胚胎成纤维细胞的硫酸盐和谷胱甘肽结合物的 HPLC。
来自鲍鱼内脏的硫酸酯酶已用于组织绿茶多酚（GTP）分析，可以将共轭形式消化为游离形式。它还被用于检测游离形式、葡糖醛酸化形式或总GTP。
Biochem/physiol Actions
硫酸酯酶水解络合物大分子（如糖胺聚糖和硫脂）的硫酸酯。它有助于调节硫酸化状态，其决定了多种生理分子的作用。
Other Notes
一单位硫酸酯酶在 pH5.0、37℃ 条件下，每小时水解 1.0 μmole p-硫酸萘基酯（30 分钟检测）。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Quercetin increased bioavailability and decreased methylation of green tea polyphenols in vitro and in vivo
Wang P, et al.
Food, 3(6), 635-642 (2012)
Sulfatases: structure, mechanism, biological activity, inhibition, and synthetic utility
Hanson SR, et al.
Angewandte Chemie (International ed. in English), 43(43), 5736-5763 (2004)
B A Merrick et al.
Carcinogenesis, 6(9), 1303-1307 (1985-09-01)
Chromatography of benzo[a]pyrene (BaP) sulfate, glucuronide and glutathione (GSH) conjugate standards were examined by h.p.l.c. on a C8 column as modified by various organic acids and solvents. Sulfate and glucuronide standards were positional isomers derived from BaP-1,3,6,7,9 phenols and BaP-GSH
Use of a Phage Display Antibody to Measure the Enzymatic Activity of the Sulfs
Uchimura K, et al.
Test, 480, 51-64 (2010)
Green Tea Polyphenols and Metabolites in Prostatectomy Tissue: Implications for Cancer Prevention
Wang P, et al.
Cancer Prevention Research (Philadelphia, Pa.), 3(8), 985-993 (2010)
实验方案
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
相关内容
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