产品名称
[KO Validated]Anti-LIN28B antibody produced in mouse, [Knockout Validated]purified monoclonal antibody, buffered aqueous solution
biological source
(Human)
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
monoclonal
form
liquid
mol wt
27084Da
species reactivity
human
technique(s)
western blot: 1:500-1:2000
isotype
IgG1, kappa
storage temp.
-10 to -25°C
target post-translational modification
unmodified
Gene Information
human ... LIN28B(389421)
Biochem/physiol Actions
This LIN28B antibody is generated from a mouse immunized with a recombinant protein of human LIN28B.
General description
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5′-GGAG-3′ motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5′-GGAG-3′ motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Physical form
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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