biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5E10, monoclonal
form
liquid
mol wt
84 kDa
species reactivity
mouse, hamster, monkey, rat, human
concentration
1 mg/mL
technique(s)
ChIP: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2b
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... THOC1(9984)
Immunogen
Amino acids 15-374 of human p84 expressed in E. coli.
Application
Recommended Starting Dilutions: For ICC/IF: Use at a concentration of 0.5 - 2 μg/mL. For IP: 1:100-1:500. For WB: Use at a concentration of 0.3 - 2 μg/mL. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.
Biochem/physiol Actions
P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Phosphate-buffered saline, no preservative added.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Luping Zhang et al.
PLoS genetics, 16(8), e1008953-e1008953 (2020-08-11)
Apoptosis of cochlear hair cells is a key step towards age-related hearing loss. Although numerous genes have been implicated in the genetic causes of late-onset, progressive hearing loss, few show direct links to the proapoptotic process. By genome-wide linkage analysis
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