biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4A10
monoclonal
form
liquid
mol wt
46 kDa
species reactivity
human, monkey, mouse, rat, hamster
concentration
1 mg/mL
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:500-1:3000
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TSG101(7251)
Application
Suggested starting dilutions are as follows: WB: 1:500-1:3000. Suggested fixation for ICC/IF: 3-3.5% PFA with 0.05% saponin treatment. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Monoclonal Anti-TSG101 antibody has been used in western blotting and immunoprecipitation (IP) analyses.
Monoclonal Anti-TSG101 antibody has been used in western blotting and immunoprecipitation (IP) analyses.
Biochem/physiol Actions
The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a luecine-zipper motif within a coiled-coil domain near the carboxyl terminus.
Tumor susceptibility gene 101 (TSG101) is essential for the generation of central supramolecular activation cluster, and the signal termination of T cell receptor (TCR) microclusters (MC). It plays an important role in TCR downregulation and separation of TCR- major histocompatibility complex (MHC)-peptide from signaling complexes, that are enriched with protein kinase C θ (PKCθ).
TSG101-4A10 recognizes TSG101 protein, the product of a recently identified tumor susceptibility gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice.
TSG101-4A10 recognizes TSG101 protein, the product of a recently identified tumor susceptibility gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
General description
Tumor susceptibility gene 101 (TSG101) is an endosomal sorting complex required for transport (ESCRT) component, present on endosomal membranes. This gene is located on chromosome 11p15. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.
Immunogen
Amino acids 167-374 of TSG101 protein expressed in E. coli.
Physical form
Phosphate-buffered saline, no preservative added.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Absence of TSG101 transcript abnormalities in human cancers.
Trink B, et al.
Oncogene, 16(21), 2815-2815 (1998)
Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia.
Zhang B, et al.
Nature Medicine, 24(4), 450-450 (2018)
Bettina Mannerström et al.
Epigenetics, 14(4), 352-364 (2019-03-26)
Extracellular vesicles (EVs) are central to intercellular communication and play an important role in cancer progression and development. Osteosarcoma (OS) is an aggressive bone tumour, characterized by the presence of malignant mesenchymal cells. The specific tumour-driving genetic alterations that are
Bin Zhang et al.
Nature medicine, 24(4), 450-462 (2018-03-06)
Leukemia stem cells (LSCs) in individuals with chronic myelogenous leukemia (CML) (hereafter referred to as CML LSCs) are responsible for initiating and maintaining clonal hematopoiesis. These cells persist in the bone marrow (BM) despite effective inhibition of BCR-ABL kinase activity
Ang Li et al.
International journal of oncology, 64(6) (2024-04-19)
The exosomal pathway is an essential mechanism that regulates the abnormal content of microRNAs (miRNAs) in hepatocellular carcinoma (HCC). The directional transport of miRNAs requires the assistance of RNA‑binding proteins (RBPs). The present study found that RBPs participate in the
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