SAB4200075
Anti-VAC14 (C-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-ArPIKfyve, Anti-TAX1-binding protein 2, Anti-TAX1BP2, Anti-TAX1BP2 TAX-reactive protein x, Anti-TRX
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
3
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~82 kDa
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μL
concentration
~1.5 mg/mL
technique(s)
immunoprecipitation (IP): 10-15 μg using A431 cell lysates, indirect immunofluorescence: 8-16 μg/mL using HeLa cells, western blot: 1-2 μg/mL using extracts of mouse brain (S1 fraction) or rat brain (S1 fraction)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VAC14(55697)
rat ... Vac14(307842)
General description
VAC14 gene is mapped to human chromosome 16q22.1-q22.2. VAC14 is a scaffold protein and is a component of the PIKfyve protein kinase complex.
Application
Anti-VAC14 (C-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
Anti-VAC14 (C-terminal) specifically recognizes human, mouse, and rat VAC14.
Mutation in VAC14 gene leads to the development of retinitis pigmentosa (RP).
VAC14 is a regulator of PIP5K3/PIKfyve, a dual specificity kinase.
VAC14 phosphorylates PtdIns(3)P to generate the housekeeping phospholipid PtdIns(3,5)P2. It associates with and upregulate PIKfyve phosphoinositide-5-kinase activity. VAC14-PIP5K3- PtdIns(3,5)P2 pathway is thought to be physiologically linked to insulin activation of glucose transport into the cell. Knockout of the VAC14 gene in mice results in massive neurodegeneration and particularly affected are neurons in the midbrain and of the peripheral sensory system.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Gholson J Lyon et al.
Cold Spring Harbor molecular case studies, 5(6) (2019-08-08)
Whole-exome sequencing was used to identify the genetic etiology of a rapidly progressing neurological disease present in two of six siblings with early childhood onset of severe progressive spastic paraparesis and learning disabilities. A homozygous mutation (c.2005G>T, p, V669L) was
ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality
Sbrissa D, et al.
Journal of Molecular Biology, 384(4), 766-779 (2008)
PIKfyve: Partners, significance, debates and paradoxes
Shisheva A
Cell Biology International, 32(6), 591-604 (2008)
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