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Merck
CN

SAB4200095

Anti-PKM2 (isoform M2) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

别名:

Anti-CTHBP, Anti-OIP3(OPA-interacting protein 3), Anti-PK3, Anti-PKM, Anti-Pyruvate Kinase, MUSCLE (isoform M1), Anti-TCB, Anti-THBP1 (thyroid hormone-binding protein, cytosolic)

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NACRES:
NA.44
UNSPSC Code:
12352203

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产品名称

Anti-PKM2 (isoform M2) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

human

concentration

~1.5 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human colon carcinoma
immunoprecipitation (IP): 2-4 μg using HT29 cell lysates
indirect immunofluorescence: 4-8 μg/mL using HeLa cells
western blot: 0.5-1.0 μg/mL using HeLa cell extracts

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PKM2(5315)

相关类别

Application

Anti-PKM2 (isoform M2) antibody produced in rabbit has been used in:
  • chromatin immunoprecipitation (chip) assay
  • western blotting
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

Anti-PKM2 (isoform M2) antibody is specific for human PKM2 (isoform M2). The product is expected to react with rat and mouse PKM2. In immunoblotting, detection of the PKM2 (isoform M2) band is specifically inhibited by the PKM2 (isoform M2) immunizing peptide.
Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis. Tumor cells have been reported to exclusively overexpress the embryonic M2 isoform. The tumor metabolome is characterized by high glycolytic turnover rate and tumor cells are able to proliferate under conditions of aerobic glycolysis, known as the Warburg effect. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr105 inhibits its activity and is common in human cancers, suggesting that Tyr105 is a critical metabolic switch in cancer cells that promotes tumorigenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pyruvate Kinase (PK) has four known isoforms, namely, L, R, M1 and M2. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues. The M2 isoform has been linked to cancer metastasis .

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

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存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
049M4776V
047M4849V
020M4775

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Sally Winther et al.
American journal of physiology. Endocrinology and metabolism, 314(3), E214-E223 (2017-11-10)
During thermogenic activation, brown adipocytes take up large amounts of glucose. In addition, cold stimulation leads to an upregulation of glycolytic enzymes. Here we have investigated the importance of glycolysis for brown adipocyte glucose consumption and thermogenesis. Using siRNA-mediated knockdown
Ying Shi et al.
Cancer letters, 422, 81-93 (2018-02-27)
Radiation therapy has become an important tool in the treatment of cancer patients, but most patients relapse within 5 years. Relapse is due to the presence of cancer stem cells (CSCs), but the molecular mechanism of radioresistance in CSCs remains
Qin Hao et al.
American journal of physiology. Endocrinology and metabolism, 308(5), E380-E392 (2014-12-18)
We applied digital gene expression profiling to determine the transcriptome of brown and white adipose tissues (BAT and WAT, respectively) during cold exposure. Male C57BL/6J mice were exposed to cold for 2 or 4 days. A notable induction of genes
Tyrosine phosphorylation inhibits PKM2 to promote the Warburg effect and tumor growth
Hitosugi T, et al.
Science Signaling, 2(97), ra73-ra73 (2009)
Prakasam Gopinath et al.
The Journal of biological chemistry (2016-04-30)
This article has been withdrawn by the authors. The PKM2 immunoblot in Fig 2E was reused as part of the Caspase-3 immunoblot in Fig 9C. The PKM2 immunoblot from 5 mM Glu, fractions 1-10 was reused as the PKM2 immunoblot
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Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

需氧糖酵解和瓦博格效应

本页面介绍了一篇有关瓦博格效应的文章，以及其如何能够在正常氧气水平下，增强肿瘤细胞中葡萄糖向乳酸的转化。Otto Heinrich Warburg在1924年证明，癌细胞显示出对糖酵解的依赖性增加，以满足他们的能量需求，无论是否有充足的氧气存在。

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