SAB4200115
Anti-Reptin antibody, Mouse monoclonal
clone 2E9-5, purified from hybridoma cell culture
别名:
Anti-CGI-46, Anti-ECP51 (Erythrocyte cytosolic protein, 51-KD), Anti-INO80J, Anti-RUVBL2 (RuvB-like 2), Anti-RVB2, Anti-TIH2, Anti-TIP48 (TATA box-binding protein-interacting protein, 48-KD), Anti-TIP49B
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
2E9-5, monoclonal
Application:
WB
Citations:
12
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
2E9-5, monoclonal
form
buffered aqueous solution
mol wt
antigen ~51 kDa
species reactivity
human, rat, mouse
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
western blot: 0.5-1.0 μg/mL using whole extract of human MCF-7 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RUVBL2(10856)
General description
Monoclonal Anti-Reptin (mouse IgG1 isotype) is derived from the hybridoma 2E9-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human Reptin fusion protein.
Reptin, also known as RuvB-like 2 RUVBL2, is encoded by the gene mapped to human chromosome 19q13.33. The encoded protein belongs to the AAA+ family of DNA helicases.
Immunogen
a human Reptin fusion protein. The corresponding sequence differs by one amino acid in mouse.
Application
Monoclonal Anti-Reptin antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
- confocal microscopy
Biochem/physiol Actions
Reptin and pontin are associated with several chromatin-remodeling complexes and are involved in multiple biological processes including chromatin remodeling, DNA damage repair, telomerase activity, transcriptional regulation, apoptosis and cancer metastasis. Both proteins are also involved in cellular transformation by β-catenin and c-myc through their chromatin remodeling function.
Reptin has been involved in various cellular processes, including the response to DNA double-strand breaks and the control of gene expression. The encoded protein plays a vital role in repression of specific β-catenin and nuclear factor-κB targets. Elevated expression of the gene leads to the development of hepatocellular carcinomas (HCC) and renal cell carcinoma (RCC). Thus, reptin can be considered as a potential therapeutic target for HCC and RCC.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Control of transcription by Pontin and Reptin.
Gallant P
Trends in Cell Biology, 17, 187-192 (2007)
Pontin and Reptin regulate cell proliferation in early Xenopus embryos in collaboration with c-Myc and Miz-1
Etard C, et al.
Mechanisms of Development, 122(4), 545-556 (2005)
RUVBL2 (RuvB-like 2 (E. coli))
Grigoletto A
Atlas of Genetics and Cytogenetics in Oncology and Haematology, 13, 257-259 (2009)
Overexpression of reptin in renal cell carcinoma contributes to tumor malignancies and its inhibition triggers senescence of cancer cells.
Ren J
Urologic Oncology, 31, 1358-1366 (2013)
Reptin and Pontin oligomerization and activity are modulated through histone H3 N-terminal tail interaction
Queval R, et al.
The Journal of Biological Chemistry, 289(49), 33999-34012 (2014)
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