SAB4200148
Anti-Derlin-1 antibody, Mouse monoclonal
clone Derlin1-1, purified from hybridoma cell culture
别名:
Anti-DER-1, Anti-DERL1, Anti-Der1-like domain family, member 1
产品名称
Anti-Derlin-1 antibody, Mouse monoclonal, clone Derlin1-1, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
Derlin1-1, monoclonal
mol wt
antigen ~22 kDa
species reactivity
mouse, bovine, rat, canine, human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): suitable
western blot: 2-4 μg/mL using whole extracts of mouse NIH-3T3 or rat NRK cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DERL1(79139)
mouse ... Derl1(67819)
rat ... Derl1(362912)
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Immunogen
synthetic peptide corresponding to the C-terminal region of human Derlin-1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, pig, bovine and canine.
Application
Monoclonal Anti-Derlin-1 antibody produced in mouse has been used in western blotting and immunoprecipitation.
Biochem/physiol Actions
Derlin-1 is a multifunctional protein. It acts as a key growth factor-responsive endothelial antigen that stimulates endothelial cell survival and growth. The encoded protein might also be implicated in endoplasmic reticulum stress in neuronal cells in Alzheimer′s disease.Elevated expression of DERL1 has been observed in various types of cancer including bladder and lung cancer. Additionally, overexpression of the gene is also associated with the development of colon cancers. Thus, DERL1 can be used as a potential therapeutic target for colon cancer. DERL1 plays an essential role in proteostasis regulation of potassium channel (KATP)channels.
Derlin-1 is required for the dislocation of misfolded proteins from the endoplasmic reticulum (ER) lumen to the cytosol. It interacts with peptide:N-glycosidase (PNGase), a deglycosylating enzyme, bringing it close to misfolding dislocating glycoproteins. Derlin-1 interacts with VIMP (VCP-interacting membrane protein), a membrane protein that recruits p97 and its cofactors, ubiquitin recognition factor in ER associated degradation 1 (Ufd1) and nuclear protein localization 4 (Npl4). Derlin-1 and VIMP form a membrane protein complex that serves as a receptor for p97.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Derlin-1 (DERL1) gene with seven exons is mapped to human chromosome 8q24.13. DERL1 is an endoplasmic reticulum (ER) membrane protein and is a key component of p97 ATPase complex.
Monoclonal Anti-Derlin-1 (mouse IgG1 isotype) is derived from the hybridoma Derlin1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Derlin-1, a human homolog of yeast Der1p, is a membrane protein.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Role of Derlin-1 protein in proteostasis regulation of ATP-sensitive potassium channels.
Wang F
The Journal of Biological Chemistry, 287, 10482-10493 (2012)
Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response and are required for ER-associated degradation
Oda Y, et al.
The Journal of cell biology, 172, 383-393 (2006)
Derlin-1 is overexpressed in human colon cancer and promotes cancer cell proliferation.
Tan X
Molecular and Cellular Biochemistry, 408, 205-213 (2015)
Derlin-1-immunopositive inclusions in patients with Alzheimer's disease.
Honjo Y
Neuroreport, 23, 611-615 (2012)
High Expression of Derlin-1 Is Associated with the Malignancy of Bladder Cancer in a Chinese Han Population.
Wu Z
PLoS ONE, 11 (2016)
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