Anti-Derlin-1 antibody, Mouse monoclonal

Derlin-1 (DERL1) gene with seven exons is mapped to human chromosome 8q24.13. DERL1 is an endoplasmic reticulum (ER) membrane protein and is a key component of p97 ATPase complex.

Monoclonal Anti-Derlin-1 (mouse IgG1 isotype) is derived from the hybridoma Derlin1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Derlin-1, a human homolog of yeast Der1p, is a membrane protein.

synthetic peptide corresponding to the C-terminal region of human Derlin-1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, pig, bovine and canine.

Monoclonal Anti-Derlin-1 antibody produced in mouse has been used in western blotting and immunoprecipitation.

Derlin-1 is a multifunctional protein. It acts as a key growth factor-responsive endothelial antigen that stimulates endothelial cell survival and growth. The encoded protein might also be implicated in endoplasmic reticulum stress in neuronal cells in Alzheimer′s disease.Elevated expression of DERL1 has been observed in various types of cancer including bladder and lung cancer. Additionally, overexpression of the gene is also associated with the development of colon cancers. Thus, DERL1 can be used as a potential therapeutic target for colon cancer. DERL1 plays an essential role in proteostasis regulation of potassium channel (KATP)channels.

Derlin-1 is required for the dislocation of misfolded proteins from the endoplasmic reticulum (ER) lumen to the cytosol. It interacts with peptide:N-glycosidase (PNGase), a deglycosylating enzyme, bringing it close to misfolding dislocating glycoproteins. Derlin-1 interacts with VIMP (VCP-interacting membrane protein), a membrane protein that recruits p97 and its cofactors, ubiquitin recognition factor in ER associated degradation 1 (Ufd1) and nuclear protein localization 4 (Npl4). Derlin-1 and VIMP form a membrane protein complex that serves as a receptor for p97.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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