Anti-WTX (N-terminal) antibody produced in rabbit

APC membrane recruitment protein 1 (AMER1) or Wilms Tumor on the X (WTX) gene, also known as FAM123B, comprises adenomatous polyposis coli (APC) interacting domains, nuclear localization signal (NLS), phospholipid binding region, six conserved block sequences and β-catenin binding region. The N-terminal region harbors the internal splicing sites (donor and acceptor). It encodes three isoforms AMER1, AMER2 and AMER3 APC, by alternative splicing at the N-terminus. WTX gene is localized on the X chromosome. The phosphatidylinositol (4,5)-bisphosphate binding site of the WTX aids its localization to the plasma membrane.

Anti-WTX (N-terminal) antibody produced in rabbit has been used in chromatin immunoprecipitation and immunoblotting.

APC membrane recruitment protein 1 (AMER1) or WTX is localized in the subnuclear compartment and participates in RNA processing, transcription and cell differentiation. In cultured cells, WTX negatively regulates the WNT/β-catenin signaling by promoting β-catenin ubiquitination and thus targets its degradation by the proteasome. WTX has been shown to form a complex with β-catenin, Axis inhibition protein 1 (AXIN1), β-transducin repeat containing protein 2 (β-TrCP2) and adenomatous polyposis coli (APC). WTX mutations are majorly somatic and the gene deletions and truncations is implicated in 30% of Wilms tumors. The mutations were confined to the active X and single X chromosomes in females and males respectively.. Loss of WTX function in Wilms tumor results in stabilization of β-catenin and its translocation to the nucleus. WTX co-localizes with non-POU domain-containing octamer-binding protein (p54nrb/NONO) and binds Wilms′ tumor protein 1 (WT1) to modulate its activity, suggesting that WTX may play a role in the transcriptional regulation.

Anti-WTX (N-terminal) specifically recognizes human WTX.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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