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安全信息

SAB4200194

Sigma-Aldrich

Anti-Pontin antibody, Mouse monoclonal

clone 5G3-11, purified from hybridoma cell culture

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别名:
Anti-49 kDa TATA box-binding protein-interacting protein, Anti-49 kDa TBP-interacting protein, Anti-54 kDa erythrocyte cytosolic protein, Anti-ECP-54, Anti-INO80 complex subunit H, Anti-NMP 238, Anti-Pontin52, Anti-RUVBL1, Anti-RVB1, Anti-RuvB (e coli homolog)-like 1, Anti-RuvB-like 1, Anti-TAP54-alpha, Anti-TIH1, Anti-TIP49, Anti-TIP49A, Anti-TIP60-associated protein 54-alpha, Anti-nuclear matrix protein 238
NACRES:
NA.41

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

5G3-11, monoclonal

形式

buffered aqueous solution

分子量

~50 kDa

种属反应性

canine, human, monkey

技术

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 1:1,000-1:2,000 using whole extracts of human HEK-293T or MCF-7 cells

同位素/亚型

IgG

UniProt登记号

Q9Y265

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... RUVBL1(8607)

一般描述

Monoclonal Anti-Pontin (mouse IgG1 isotype) is derived from the hybridoma 5G3-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human pontin fusion protein. Pontin and reptin are two related members of the AAA+ (ATPases associated with diverse cellular activities) family of ATPases. Pontin and reptin share some homology to the bacterial RuvB gene.

免疫原

human Pontin fusion protein

应用

Monoclonal Anti-Pontin antibody produced in mouse has been used in:
  • western blotting
  • immunoprecipitation
  • ChIP-sequencing
  • immunohistochemistry
  • immunofluorescence
  • RNA immunoprecipitation

生化/生理作用

Pontin and Reptin are associated with several chromatin-remodeling complexes and are involved in multiple biological processes including chromatin remodeling, DNA damage repair, transcriptional regulation, telomerase activity, apoptosis and cancer metastasis. Both proteins are also involved in cellular transformation by beta-catenin and c-myc through their chromatin remodeling function.

外形

Solution in 0.01 M phosphate buffered saline, 7.4 pH, containing 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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A novel interaction of ecdysoneless (ECD) protein with R2TP complex component RUVBL1 is required for the functional role of ECD in cell cycle progression
Mir RA, et al.
Molecular and Cellular Biology, 36(6), 886- 899 (2016)
The Emergence of the Conserved AAA+ ATPases Pontin and Reptin on the Signaling Landscape
Rosenbaum J, et al.
Science Signaling, 6(266), mr1-mr1 (2013)
Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells
Boo K, et al.
Nature Communications, 6810-6810 (2015)
Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression
Tarangelo A, et al.
Nature Communications, 6(6), 10028-10028 (2015)
Gabrijela Dumbovic et al.
Nucleic acids research, 46(11), 5504-5524 (2018-06-19)
Primate-specific NBL2 macrosatellite is hypomethylated in several types of tumors, yet the consequences of this DNA hypomethylation remain unknown. We show that NBL2 conserved repeats are close to the centromeres of most acrocentric chromosomes. NBL2 associates with the perinucleolar region
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