SAB4200195
Anti-Brg1 antibody, Rat monoclonal
clone 5B7, purified from hybridoma cell culture
别名:
Anti-BAF190, Anti-RTPS2, Anti-SMARCA4, Anti-SNF2, Anti-SNF2-BETA, Anti-SNF2L4, Anti-SNF2LB, Anti-SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4, Anti-SWI2, Anti-hSNF2b, Monoclonal Anti-Brg1 antibody produced in rat
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
5B7, monoclonal
Application:
ChIP, ICC, IP, WB
Citations:
10
biological source
rat
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
5B7, monoclonal
form
buffered aqueous solution
mol wt
antigen ~185 kDa
species reactivity
mouse, monkey, human
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.5-1.0 μg/mL using HeLa, HEK-293T, Raji or HepG2 cells extracts, ChIP: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SMARCA4(6597)
mouse ... Smarca4(20586)
General description
Brahma-related gene 1 (Brg1), also known as SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), is encoded by the gene mapped to human chromosome 19p13.2. The encoded protein is characterized with a conserved catalytic ATPase domain, a conserved C-terminal bromodomain, AT-hook motif and the less characterized N-terminal region housing QLQ, helicase-SANT-associated (HSA) and BRM and KIS (BRK) domains.
Monoclonal Anti-Brg1 (rat IgG2a isotype) is derived from the hybridoma 5B7 produced by the fusion of mouse myeloma cells (SP2) and splenocytes from rat immunized with a fusion protein corresponding to a fragment of human Brg1. Brahma-related gene 1 (Brg1) catalytic subunit of mammalian SWItch/sucrose non-fermentable (SWI/SNF)-related complexes co-localizes with origin recognition complexes, GINS complexes and proliferating cell nuclear antigen.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Monoclonal Anti-Brg1 antibody produced in rat has been used in:
- immunoblotting
- immunocytochemistry
- immunoprecipitation
- chromatin immunoprecipitation
Biochem/physiol Actions
Brahma-related gene 1 (Brg1) acts as a transcriptional repressor. It interacts with retinoblastoma tumor suppressor to form a repressor complex which inhibits cell cycle proteins such as cyclins A, D1 and E. Brg1 plays an important role as tumor suppressor to maintain genome stability and prevent cancer.
Brahma-related gene 1 (Brg1), acts as a catalytic subunit for various mammalian SWItch/Sucrose non-fermentable (SWI/SNF) complexes, where it helps in transcription regulation through the nucleosome remodelling. Additionally, it plays an essential role in differentiation of myeloid to granulocytes. Mutation in the gene is associated with the development of Coffin-Siris syndrome and rhabdoid tumor predisposition syndrome. Loss of Brg1 expression is used as a potential diagnostic marker for ovarian small cell carcinoma of the hypercalcemic type (SCCOHT).
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Germline nonsense mutation and somatic inactivation of SMARCA4/BRG1 in a family with rhabdoid tumor predisposition syndrome.
Schneppenheim R
American Journal of Human Genetics, 86, 279-284 (2010)
The BRG1 transcriptional coregulator
Trotter KW and Archer TK.
Nuclear Receptor Signaling (2008)
SMARCA4 (BRG1) loss of expression is a useful marker for the diagnosis of ovarian small cell carcinoma of the hypercalcemic type (ovarian rhabdoid tumor): a comprehensive analysis of 116 rare gynecologic tumors, 9 soft tissue tumors, and 9 melanomas.
Karanian-Philippe M
American Journal of Surgical Pathology, 39, 1197-1205 (2015)
Mutations affecting components of the SWI/SNF complex cause Coffin-Siris syndrome.
Tsurusaki Y
Nature Genetics, 44, 376-378 (2012)
BRG1 variant rs1122608 on chromosome 19p13.2 confers protection against stroke and regulates expression of pre-mRNA-splicing factor SFRS3
Xiong X
Human Genetics, 133, 499-508 (2014)
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