SAB4200235
Anti-TGN46 antibody, Mouse monoclonal
clone TGN46-52, purified from hybridoma cell culture
别名:
Anti-Trans-Golgi network protein, 46-kD
产品名称
Anti-TGN46 antibody, Mouse monoclonal, clone TGN46-52, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
TGN46-52, monoclonal
form
buffered aqueous solution
mol wt
antigen 80-100 kDa
species reactivity
human
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human TGN46
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TGN46(10618)
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Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-TGN46 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Trans-Golgi network protein, 46-kDa (TGN46) is involved in regulating membrane traffic to and from the TGN. It cycles constitutively between the TGN and the plasma membrane, returning via endosomes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-TGN46 (mouse IgG1 isotype) is derived from the hybridoma TGN46-52 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human TGN46 conjugated to KLH. Trans-Golgi network protein 46-kDa (TGN46), It is a heavily glycosylated protein, containing a signal peptide, a lumenal domain, a membrane-spanning domain and a cytoplasmic domain. The membrane spanning region and the cytoplasmic tail contain the retention and retrieval signals for localization in the TGN.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Haik Mkhikian et al.
eLife, 5 (2016-06-09)
Essential biological systems employ self-correcting mechanisms to maintain cellular homeostasis. Mammalian cell function is dynamically regulated by the interaction of cell surface galectins with branched N-glycans. Here we report that N-glycan branching deficiency triggers the Golgi to generate bioequivalent N-glycans
Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network
Liljedahl M, et al.
Cell, 104(3), 409-420 (2001)
Golgi self-correction generates bioequivalent glycans to preserve cellular homeostasis
Mkhikian H, et al.
eLife, 5(20), e14814-e14814 (2016)
A new class of carriers that transport selective cargo from the trans Golgi network to the cell surface
Wakana Y, et al.
The Embo Journal, 31(20), 3976-3990 (2012)
Maorong Chen et al.
eLife, 9 (2020-09-15)
Wnt signaling through the Frizzled (FZD) family of serpentine receptors is essential for embryogenesis and homeostasis, and stringent control of the FZD protein level is critical for stem cell regulation. Through CRISPR/Cas9 genome-wide screening in human cells, we identified TMEM79/MATTRIN
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