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Merck
CN

SAB4200527

Anti-USP8 antibody, Mouse monoclonal

clone US872, purified from hybridoma cell culture

别名:

Anti-Deubiquitinating enzyme 8, Anti-HumORF8, Anti-UBPY, Anti-Ubiquitin carboxyl-terminal hydrolase 8, Anti-Ubiquitin isopeptidase Y, Anti-Ubiquitin specific peptidase 8, Anti-Ubiquitin thioesterase 8, Anti-Ubiquitin-specific-processing protease 8, Anti-hUBPy, Monoclonal Anti-USP8 antibody produced in mouse

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
US872, monoclonal
Application:
flow cytometry
indirect immunofluorescence
western blot
Species reactivity:
human, rat, bovine, monkey, mouse, dog
Citations:
7
Technique(s):
flow cytometry: 5-10 μg/test using MCF7 cells
indirect immunofluorescence: 1.25-2.5 μg/mL using A549 cells
western blot: 0.5-1.0 μg/mL using MCF7 total cell extracts
Uniprot accession no.:
P40818

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产品名称

Anti-USP8 antibody, Mouse monoclonal, clone US872, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

US872, monoclonal

form

buffered aqueous solution

mol wt

antigen ~127 kDa
antigen ~70 kDa

species reactivity

human, rat, bovine, monkey, mouse, dog

concentration

~1.0 mg/mL

technique(s)

flow cytometry: 5-10 μg/test using MCF7 cells
indirect immunofluorescence: 1.25-2.5 μg/mL using A549 cells
western blot: 0.5-1.0 μg/mL using MCF7 total cell extracts

UniProt accession no.

P40818

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... USP8(9101)

相关类别

Application

Monoclonal Anti-USP8 antibody produced in mouse has been used in:immunoblotting immunofluorescence,flow cytometry, immunocytochemistry.

Biochem/physiol Actions

Ubiquitin specific protease 8 (USP8) was found to regulate ubiquitination of frizzled, a receptor for Wnt/Wingless. Deletion of USP8 in mice causes embryonic lethality. The defect was accompanied by a strong reduction or absence of several growth factor receptor tyrosine kinases (RTKs), like EGFR, hepatocyte growth factor receptor (c-met), and ERBB3.USP8 inactivation results in enhanced ubiquitination of ligand-activated EGFR. USP8 in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect epidermal growth factor receptor (EGFR) as well as other receptors from lysosomal degradation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-USP8 (mouse IgG1 isotype) is derived from the hybridoma US872 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Ubiquitin specific protease 8 (USP8) belongs to the family of de-ubiquitinating enzymes (DUBs).

Immunogen

synthetic peptide corresponding to an internal sequence of human USP8, conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000188288
0000141096
0000188288
097M4793V

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Balanced ubiquitylation and deubiquitylation of Frizzled regulate cellular responsiveness to Wg/Wnt
Mukai A, et al.
The Embo Journal, 29(13), 2114-2125 (2010)
Essential Role of Ubiquitin-Specific Protease 8 for Receptor Tyrosine Kinase Stability and Endocytic Trafficking In Vivo
Niendorf S, et al.
Molecular and cellular biology, 27(13), 5029-5039 (2007)
The ubiquitin-specific protease USP8 deubiquitinates and stabilizes Cx43
Sun , et al.
The Journal of biological chemistry, 293(21), 8275-8284 (2018)
Jian Sun et al.
The Journal of biological chemistry, 293(21), 8275-8284 (2018-04-08)
Connexin-43 (Cx43, also known as GJA1) is the most ubiquitously expressed connexin isoform in mammalian tissues. It forms intercellular gap junction (GJ) channels, enabling adjacent cells to communicate both electrically and metabolically. Cx43 is a short-lived protein which can be
Haifeng Gu et al.
Nature communications, 10(1), 1465-1465 (2019-04-02)
Embryonic stem cells (ESCs) can propagate in an undifferentiated state indefinitely in culture and retain the potential to differentiate into any somatic lineage as well as germ cells. The catabolic process autophagy has been reported to be involved in ESC
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